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Jaeyoung Kim, Yun Hee Kim, Dong Won Hyun, Eun Jung Shin, Ah Young Koh, Hyun Ju Lee, DongHyun Kim, Won Ryang Wee, Saewha Jeon, Mee Kum Kim; Comparative analysis of substrate-free cultured oral mucosal epithelial cell sheets from cells of subjects with and without Stevens-Johnson syndrome for use in ocular surface reconstruction. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3454.
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© ARVO (1962-2015); The Authors (2016-present)
To treat limbal deficiency, cultured oral mucosal epithelial cell sheets (COMECs) have recently been explored; however, the properties of oral mucosal epithelial cells from Stevens-Johnson syndrome (SJS) subjects are not well characterized. The purpose of this study was to compare the regenerative potential of cultured oral mucosal epithelial cells from SJS and non-SJS subjects.
Human oral mucosal epithelial cells from SJS and non-SJS subjects were cultured, and colony-forming efficiency (CFE), proliferative and migration potential, expression of cytokeratin, stemness (p63, Ki-67), and cytokines/growth factors were compared. COMECs from SJS and non-SJS subjects were transplanted to 12 limbal-deficient rabbits. Survival and characteristics of cytokeratin/stemness were compared at 1 week after transplantation.
Mean CFE, proliferation potential, and stemness in mucosal epithelial cells from SJS subjects were similar to those in the cells from non-SJS subjects, while migratory potential was significantly delayed in the cells from SJS subjects. The SJS cells expressed lower levels of matrix metalloproteinase (MMP)-2 and MMP-9 and higher levels of vascular endothelial growth factor compared to the non-SJS cells. SJS COMECs showed similar expression of cytokeratin, proliferation, and stem cell markers compared to those from non-SJS subjects in limbal deficiency models. The initial epithelial defects were larger in the eyes treated with SJS COMECs at day 3, but no differences were observed by day 7.
The results suggest that, aside from differences in migratory potential, oral mucosal epithelial cells from SJS and non-SJS subjects are comparable in their potential use in treating limbal deficiency.
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