June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Comparative analysis of substrate-free cultured oral mucosal epithelial cell sheets from cells of subjects with and without Stevens-Johnson syndrome for use in ocular surface reconstruction
Author Affiliations & Notes
  • Jaeyoung Kim
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
    Laboraory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea (the Republic of)
  • Yun Hee Kim
    Cutigen Research Institute, Tego Science Inc., Seoul, Korea (the Republic of)
  • Dong Won Hyun
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
    Laboraory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea (the Republic of)
  • Eun Jung Shin
    Cutigen Research Institute, Tego Science Inc., Seoul, Korea (the Republic of)
  • Ah Young Koh
    Laboraory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea (the Republic of)
  • Hyun Ju Lee
    Laboraory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea (the Republic of)
  • DongHyun Kim
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
    Laboraory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea (the Republic of)
  • Won Ryang Wee
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
    Laboraory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea (the Republic of)
  • Saewha Jeon
    Cutigen Research Institute, Tego Science Inc., Seoul, Korea (the Republic of)
  • Mee Kum Kim
    Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
    Laboraory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships Jaeyoung Kim, None; Yun Hee Kim, Cutigen Research Institute, Tego Science Inc. (E); Dong Won Hyun, None; Eun Jung Shin, Cutigen Research Institute, Tego Science Inc. (E); Ah Young Koh, None; Hyun Ju Lee, None; DongHyun Kim, None; Won Ryang Wee, None; Saewha Jeon, Cutigen Research Institute, Tego Science Inc. (E); Mee Kum Kim, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3454. doi:
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      Jaeyoung Kim, Yun Hee Kim, Dong Won Hyun, Eun Jung Shin, Ah Young Koh, Hyun Ju Lee, DongHyun Kim, Won Ryang Wee, Saewha Jeon, Mee Kum Kim; Comparative analysis of substrate-free cultured oral mucosal epithelial cell sheets from cells of subjects with and without Stevens-Johnson syndrome for use in ocular surface reconstruction. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3454.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To treat limbal deficiency, cultured oral mucosal epithelial cell sheets (COMECs) have recently been explored; however, the properties of oral mucosal epithelial cells from Stevens-Johnson syndrome (SJS) subjects are not well characterized. The purpose of this study was to compare the regenerative potential of cultured oral mucosal epithelial cells from SJS and non-SJS subjects.

Methods: Human oral mucosal epithelial cells from SJS and non-SJS subjects were cultured, and colony-forming efficiency (CFE), proliferative and migration potential, expression of cytokeratin, stemness (p63, Ki-67), and cytokines/growth factors were compared. COMECs from SJS and non-SJS subjects were transplanted to 12 limbal-deficient rabbits. Survival and characteristics of cytokeratin/stemness were compared at 1 week after transplantation.

Results: Mean CFE, proliferation potential, and stemness in mucosal epithelial cells from SJS subjects were similar to those in the cells from non-SJS subjects, while migratory potential was significantly delayed in the cells from SJS subjects. The SJS cells expressed lower levels of matrix metalloproteinase (MMP)-2 and MMP-9 and higher levels of vascular endothelial growth factor compared to the non-SJS cells. SJS COMECs showed similar expression of cytokeratin, proliferation, and stem cell markers compared to those from non-SJS subjects in limbal deficiency models. The initial epithelial defects were larger in the eyes treated with SJS COMECs at day 3, but no differences were observed by day 7.

Conclusions: The results suggest that, aside from differences in migratory potential, oral mucosal epithelial cells from SJS and non-SJS subjects are comparable in their potential use in treating limbal deficiency.

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