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Koju Kamoi, ZHAORONG GUO, Shintaro Horie, Kyoko Ohno-Matsui; The role of HTLV-1 infected RPE cells in the pathogenesis of HTLV-1 uveitis. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3495.
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© ARVO (1962-2015); The Authors (2016-present)
Human T-cell lymphotropic virus-1 (HTLV-1) infection affects ocular tolerance, which causes various eye diseases. Most studies have not focused on the effect of HTLV-1 infection to ocular tissues, although the infection has an intimate involvement in HTLV-1 related eye diseases. In this study, we investigate the inflammatory change that caused by HTLV-1 infection to the retinal epithelium (RPE) cells.
HTLV-1 producing T cell line (MT2) and RPE cell line (ARPE-19) were co-cultured via direct contact or indirect contact methods. The efficiency of HTLV-1 infection was determined by real-time PCR. The production of inflammatory cytokines/ chemokines was measured by Cytometric Beads Array.
In RPE cells, HTLV-1 infection could be established even through indirect contact with HTLV-1 producing T cells. Induction of IL-6 and IL-8 were seen in both direct contact and indirect contact infection, but IL-10, TNF-alpha, IL-1β, and IL-12p70 were not secreted. Chemokines such as MCP-1 and RANTES were detected in direct contact and indirect contact, but IL-10 production was seen only through direct contact.<br /> <br />
HTLV-1 infection to the RPE cells induces inflammatory cytokines and chemokines, which can result in intraocular inflammation. This mechanism may contribute to the pathogenesis of HTLV-1 uveitis.
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