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Wenbin Huang, Shida Chen, Jiawei Wang, Minwen Zhou, Xiulan Zhang; Effect of Intravitreal Ranibizumab on Intraocular Concentrations of angiogenesis-related factor in Neovascular Glaucoma Eyes. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3653.
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To evaluate concentrations of vascular endothelial growth factor (VEGF)-A, placental growth factor (PlGF), and pigment epithelium-derived factor (PEDF) in aqueous humor of patients with neovascular glaucoma prior to, and shortly after, an intravitreal ranibizumab injection.
The prospective interventional comparative study involved 20 eyes of 20 patients with surgery-required advanced NVG and 20 control subjects. Aqueous humor was collected at the time of the IVR injection (pre-IVR) and at the time of anti-glaucoma surgery (post-IVR). Aqueous humor was also collected at the time of cataract surgery in control subjects. The levels of VEGF-A, PlGF and PEDF in aqueous humor and plasma were measured with ELISA.
The mean aqueous humor VEGF-A, PlGF and PEDF concentrations in the pre-IVR eyes were significantly higher than in those of the control subjects (all p<0.001), whereas the plasma levels showed no significant difference. There was a statistically significant correlation between the aqueous humor PlGF and the VEGF-A concentration (r=0.612, p=0.003). The mean aqueous humor PlGF in the post-IVR eyes dramatically decreased from 1704.83 ± 757.82 to 19.02 ± 14.65 pg/mL (p<0.001). The VEGF-A level showed a similar decreased trend from 3697.64 ± 2104.47 pg/mL to 183.54 ± 130.35 pg/mL (p<0.001). The PEDF concentration remained unchanged (19±12ug/ml).
Aqueous humor concentrations of VEGF-A, PlGF and PEDF were significantly elevated in the eyes with NVG, within one week after intravitreal injection of ranibizumab, VEGF-A and PlGF concentration decreased to subnormal levels, while the PEDF concentration remained unchanged.<br /> <br />
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