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Diana Gabriela Ponce-Angulo, Antonio Bautista-Hernandez, Gerardo Aparicio-Ozores, Victor Manuel Bautista; Metaproteomic analysis in infected ocular surface. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4069.
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© ARVO (1962-2015); The Authors (2016-present)
To realize a comparative metaproteomic analysis of the ocular surface in patients with ocular infection.
The samples were obtained from patients with healthy and infected ocular surface. The human tears samples were taken following the internal protocols in both groups. Steril saline solution was applied over the ocular surface and collected by capillar tubes. Microorganisms were identified by automated microbiology methods. Chocolate agar, blood agar, Sabouraud agar were seeded with samples from both groups, samples also were added to Brain Heart Infusion. The bacterial identification was determined by Vitek 2 Compact System. The fungi were identificated by morphologycal caracteristics. The tears samples of each patient were analysed by 2D electrophoresis and differential protein expression between groups was performed using Dymensión 2 Software.
Microbiological analysis showed the presence of Staphylococus aureus, S. epidermidis, S. lentus, Kokuraria rosea, Streptococcus thoraltensis, Rothia mucilaginosa, Granulicatella adiacens and Candida guillermondi in healthy group; S. capitis, S. epidermidis and C. albicans were isolated and identified in infected group, three patients not shown microbiology growth. Proteomic analysis showed<br /> In the results of healthy group, there were differential growt of Staphylococus aureus, S. epidermidis, S. lentus , Kokuraria rosea, Streptococcus thoraltensis, Rothia mucilaginosa, Granulicatella adiacens and Candida guillermondi. In the infection group, the findings in the isolated microorganism were S. capitis, S. epidermidis and C. albicans and in three of all them had not microbiology grown.<br /> At the end of the analysis of differential expression in 2D gels without infection group compared with the control group the following results: in the first sample were obtained 5 differentially expressed spots, in the second sample 4 spots, en the third sample 2 spots, in the fourth sample 3 spots, in the fifth sample 2 spots and finally in the sixth sample 2 spots. The metaproteomic analysis shows the relevant expression of diferential proteins. The diferential proteins will be identificated by mass spectrometry.
The bacterial species were obtained in both groups were Staphylococcus epidermidis and Staphylococcus capitis. The analysis have difencial protein expression in helthy group in compararising with infection group.
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