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Berna Akova Budak, Sertac Argun Kivanc, Meral Yildiz, Merih Kivanc, Gulay Gullulu; Comparison of two different evaluation methods for biofilm formation of S.epidermidis isolated from ocular surface.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4076.
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© ARVO (1962-2015); The Authors (2016-present)
To compare of two different evaluation methods for biofilm formation of Staphylococcus epidermidis isolated from ocular surface.
S.epidermidis strains that were isolated from ocular surface previously were studied. Microtiter plate method (MPA) and Congo red agar (CRA) method were used for measuring biofilm formation. Ica A, ica D, bap genes positivity and multi-antibiotic resistance were determined. Accurracy of measuring biofilm production capacity of MPA and CRA were compared.
8 of the strains produced strong biofilm according to MPA method, and 4 of these strains also produced strong biofilm according to CRA method. 8 strains produced strong biofilm according to CRA method. 11 strains were biofilm negative with MPA however 7 (64 %) of these were positive with CRA. 15 strains were resistant to 3 or more antibiotics, 7 (46%) and 6 (40 %) of these strains produced strong biofilm according to MPA and CRA methods respectively. 8 strains were resistant to 4 or more antibiotics, 6 (75 %) and 3 (37 %) of these strains produced strong biofilm according to MPA and CRA methods respectively.
Strains that produced strong biofilm with MPA had more multi-antibiotic resistance than with CRA.These two methods may not be consistent with each other.
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