June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Structural and functional retinal imaging with MHz Line-field parallel swept source imaging (LPSI)
Author Affiliations & Notes
  • Daniel Fechtig
    Center for Med. Phys. and Biom. Eng., Medical University Vienna, Vienna, Austria
    Center for Med. Phys. and Biom. Eng., Christian Doppler Society, Vienna, Austria
  • Tilman Schmoll
    3Carl Zeiss Meditec, Inc., Dublin, CA
  • Cedric Blatter
    Center for Med. Phys. and Biom. Eng., Medical University Vienna, Vienna, Austria
  • Rainer A Leitgeb
    Center for Med. Phys. and Biom. Eng., Medical University Vienna, Vienna, Austria
    Center for Med. Phys. and Biom. Eng., Christian Doppler Society, Vienna, Austria
  • Footnotes
    Commercial Relationships Daniel Fechtig, None; Tilman Schmoll, None; Cedric Blatter, None; Rainer Leitgeb, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4092. doi:
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    • Get Citation

      Daniel Fechtig, Tilman Schmoll, Cedric Blatter, Rainer A Leitgeb; Structural and functional retinal imaging with MHz Line-field parallel swept source imaging (LPSI). Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4092.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

MHz OCT allows mitigating undesired influence of motion artifacts during retinal assessment, but comes in state-of-the-art point scanning OCT at the price of increased system complexity. By changing the paradigm from scanning to parallel OCT for in vivo retinal imaging the three-dimensional (3D) acquisition time is reduced without a trade-off between speed, sensitivity and technological requirements.

 
Methods
 

Line field parallel interferometric imaging (LPSI) is utilizing a commercially available swept source, a single-axis galvo-scanner and a line scan camera for recording 3D data with up to 1MHz A-scan rate. Besides line-focus illumination and parallel detection, we mitigate the necessity for high-speed sensor and laser technology by holographic full-range imaging, which allows for increasing the imaging speed by low sampling of the optical spectrum. High B-scan rates up to 1kHz further allow for implementation of lable-free optical angiography in 3D by calculating the inter B-scan speckle variance.

 
Results
 

We achieve a detection sensitivity of 93.5 (96.5) dB at an equivalent A-scan rate of 1 (0.6) MHz. Fig. 1(a) was obtained by stitching 7 slightly overlapping non-averaged tomograms. The sensitivity and resolution are high enough to visualize the external limiting membrane (ELM), and to contrast the various layers of the inner retina. Despite the loss of confocality in one lateral dimension and the shorter center wavelength of 840nm, the signal intensity from the choroid is remarkably strong maintaining structural details. In Fig. 1(b) we feature a enface projection of micro-angiographic information. One single 3D stack corresponds to a lateral FOV of approx. 5-6° and is acquired in just 2.5 s.

 
Conclusions
 

Our results demonstrate for the first time competitive imaging sensitivity, resolution and speed with a parallel OCT modality. LPSI is in fact currently the fastest OCT device applied to retinal imaging and operating at a central wavelength window around 800 nm with a detection sensitivity of higher than 93.5 dB.  

 
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