June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Sustained Release Dexamethasone Implants for In Vitro - In Vivo Correlations
Author Affiliations & Notes
  • YUNPENG BAI
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO
  • David Bourne
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO
  • yan wang
    U. S. Food and Drug Administration, Silver Spring, MD
  • Stephanie Choi
    U. S. Food and Drug Administration, Silver Spring, MD
  • Uday B Kompella
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO
  • Footnotes
    Commercial Relationships YUNPENG BAI, None; David Bourne, None; yan wang, None; Stephanie Choi, None; Uday Kompella, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4154. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      YUNPENG BAI, David Bourne, yan wang, Stephanie Choi, Uday B Kompella; Sustained Release Dexamethasone Implants for In Vitro - In Vivo Correlations. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4154.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To prepare qualitatively (Q1) and quantitatively (Q2) identical dexamethasone-poly(lactic-co-glycolic acid) (PLGA) implants with variable diameters and mechanical properties in order to identify implants suitable for developing in vitro-in vivo correlations.

Methods: Powders of dexamethasone and PLGA-503H were mixed at a ratio of 1:4 and implants with different diameters were manufactured with Telfon molds and warm compression. Blade cutting mode of the Texture Analyzer was used to measure the firmness and toughness of implants. Dexamethasone content in implants was quantified using a QTRAP 4500 LC-MS instrument.

Results: Warm compression followed by cooling of the molds allowed easy removal of the implants from the mold using a stylus. Dexamethasone loaded implants of diameters about 1 and 0.8 mm with a drug content of about 20 and 17%, respectively, were made. Implants with the larger diameter exhibited higher hardness (1074.6 vs 344.1 g) and toughness (108.7 vs 31.4 g•sec).

Conclusions: Using Teflon molds and warm compression, dexamethasone-PLGA implants of various diameters could be prepared. Future studies will involve preparation of implants with low, medium, and high in vitro release rates for in vivo studies and the development of in vitro-in vivo correlations.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×