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Kazuhiko Umazume, Hiroki Takahashi, Naoyuki Yamakawa, Hiroshi Goto; The role of Decorin in experimental proliferative vitreoretinopathy model. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4241.
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The cytokine transforming growth factor-ß (TGF-ß) plays a major role to tissue fibrosis. Decorin, a naturally occurring TGF-ß inhibitor, can bind activated TGF-ß and suppress TGF-ß activity. We sought to invesitigate the role of decorin in experimental vitreoretinopathy and to develop novel methods of prevention.
The effect of decorin on retinal pigment epithelial (RPE) sheet growth was determined by measuring enlargement of cultured RPE sheets in the presence or absence of decorin. Epithelial-mesenchymal transition (EMT) of RPE cells was assessed by expression of S100A4 and alpha smooth actin expression (αSMA). Type I collagen contraction assay were utilized to examine the effect of decorin on extracellular matrix (ECM) contraction.
Decorin did not prevent the growth of RPE sheets and EMT, however, suppressed the expression of αSMA in RPE sheets. Moreover, 150μg/ml of decorin inhibited nearly 70% of collagen gel contraction.
Decorin significantly inhibited PVR-related RPE changes in vitro. Theses data suggested that decorin may be effective in the prevention of PVR.
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