Expression of SIRT1 and Related Oxidative Stress in Diabetic Dry Eye Mouse

Abstract

 

Purpose

 

Observing the change of physiology and pathology in the cornea of diabetic dry eye and exploring the expression of SIRT1 and oxidative stress reaction in pathological process of diabetic dry eye.

 

Methods

 

Forty-eight C57BL/6Jdb/db mice of eight-week old were divided randomly into two groups: diabetic dry eye group (Group A, n=24) and diabetic group (Group C, n=24). Forty-eight C57BL/6J mice of eight-week old were divided randomly into another two groups: dry eye group (Group B, n=24) and control group (Group D, n=24). After dry eye models were induced at 1^st, 4^th and 8^th week, blood glucose test and aqueous tear production were measured. Its score of corneal fluorescein staining were estimated and the histopathology were also performed. Expression of SIRT1, FOXO3 and MnSOD were detected by western blot.

 

Results

 

After induction of 1^st, 4^th and 8^th week, blood glucose remained high and stable concentration in group A and group C. Significant decreased tear volume and the increased scores of corneal fluorescein stainings were found in group A, B and C (P<0.05, vs control) ( Figure 1). The least tear volume and the highest score of corneal fluorescein staining were detected in group A (P<0.05) (Figure 1). Compared to control group D, the expression of SIRT1, FOXO3 and MnSOD were higher in the 1^st week and 4^th week (P<0.05), but significantly down-regulated in the 8^th week in group A and C (P<0.05) (Figure 2). A lower expression of SIRT1,FOXO3 and MnSOD was detected in all the 1^st, 4^th and 8^th week in group B (P<0.05) (Figure 2).

 

Conclusions

 

In the induction of diabetic dry eye, tear volume decreased obviously, coupled with serious wounded corneal epithelium. The oxidative stress of cornea was enhanced significantly with its expression of SIRT1 decreased in the progression of diabetic dry eye.