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Joaquín Arturo Quiroz, Norma Ramírez-Velázquez, Yonathan Garfias; Tissue and cellular characterization of nucleolin in a murine model of corneal angiogenesis.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4496.
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© ARVO (1962-2015); The Authors (2016-present)
Nucleolin initially described as a nuclear protein, has been identified in the surface of endothelial cells of blood vessels where it participates in neo-vessels formation. To study a possible role of nucleolin in the development of blood vessels in the cornea, the aim of this work was to determine the nucleolin presence in corneal tissue with and without angiogenesis.
Following ARVO guideliness for animal research in visual science, suture induced corneal angiogenesis was performed in both BALB/c and C57BL/6 mouse strains. After clinical analysis, the corneal tissues were obtained at different time points and co-immunofluorescence assays were performed using different cellular compartments reference proteins. Control tissues were obtained from non-injured corneas.
Nucleolin was observed in the nuclear compartment, principally inside the nucleoli of certain epithelial cells of the basal layer and lesser extent in few stromal cells in both mouse strains under healthy conditions. Interestingly, angiogenesis induced considerable changes in tissue as well as cellular localization of nucleolin, the nucleolin presence was detected in the cytoplasmic compartment and the cell membrane of epithelial cells, stromal cells, and corneal endothelial cells.
Nucleolin mobilization to the cell membrane during angiogenesis reveals a possible role as a receptor of proangiogenic molecules in the corneal tissue. These results suggest that nucleolin could be a target of study and treatment of corneal angiogenesis.<br /> <br /> Financial Support: This project was supported by CONACYT: SALUD 160286; CIENCIA BASICA 167438; DGAPA-PAPIIT IA203514; CVU: 315182
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