Abstract
Purpose:
To examine mRNA expression level of angiogenic growth factors, VEGF and TGFb1, in macrophages and ocular fibroblasts derived from a tenascin X-null mouse. We previously reported that the loss of tenascin X suppress neocvascularization with reduction of in vivo expression of angiogenic growth factors in a mouse cornea (ARVO 2014).
Methods:
Peritoneal macrophages were obtained from tenascin X-null and wild types mice by using macrophage induction by oyster glycogen i.p. injection. Ocular fibroblasts were cultured from eye-shells of post-natal day 1 or 2 mice. The cultures were maintained for 24 hrs with or without exogenous TGFb1 and processed for RNA extraction. Real-time RT-PCR was ran to examine the expression level of VEGF and TGFb1.
Results:
Loss of tenascin X supprsss mRNA expression of VEGF in macrophages and of TGFb1 in fibroblasts in the absence of TGFb1. Loss of tenascin X did not attenuate the TGFb1 induction of VEGF and TGFb1 in these cell types.
Conclusions:
Tenascin X is involved in expression of angiogenic growth factors in macrophages and fibroblasts, partially explain the mechanism of inhibition of in vivo neovascularization in a tenacin X-null mouse cornea.