June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
The inhibitory effect of Itraconazole on Corneal neovascularization in Rabbits
Author Affiliations & Notes
  • Kyung Euy Hong
    Catholic University of Korea College of Medicine, Seoul, Korea, Seoul, Korea (the Republic of)
  • Jae Yon Won
    Catholic University of Korea College of Medicine, Seoul, Korea, Seoul, Korea (the Republic of)
  • Sung Kun Chung
    Catholic University of Korea College of Medicine, Seoul, Korea, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships Kyung Euy Hong, None; Jae Yon Won, None; Sung Kun Chung, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4507. doi:
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      Kyung Euy Hong, Jae Yon Won, Sung Kun Chung; The inhibitory effect of Itraconazole on Corneal neovascularization in Rabbits. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4507.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To evaluate the inhibitory effect of itraconazole on corneal neovascularization in rabbits.

Methods: Corneal neovascularization was induced in 36 eyes of 18 rabbits by suture placement in the corneal stroma. Seven days after suture placement, all rabbits were randomly divided into 4 groups and were treated four times daily with balanced salt solution (Group 1, 4 rabbits), topical 0.5% itraconazole (5 mg/mL , Group 2, 4 rabbits), topical 1% itraconazole (10 mg/mL , Group 3, 6 rabbits), and topical 2% itraconazole (20 mg/mL , Group 4, 4 rabbits). After one week, the surface area of corneal neovascularization was assessed on the digital photographs. In the corneal specimens, the concentration of VEGF A (vascular endothelial growth factor), VEGF R2, and PLGF (placental growth factor) mRNAs was measured by RT-PCR, and the concentration of ERK, p-ERK, Flk, and p-Flk was measured by Western Blotting.

Results: The surface area of induced corneal neovascularization was significantly smaller in Groups 2, 3, and 4 compared to the control group on day 14 (p<0.05). RT-PCR analysis showed that the mean concentration of VEGF and PLGF in Groups 2, 3, and 4 was significantly lower than that in the control group after 7 days of treatment. Western Blotting analysis showed that the mean concentration of p-ERK, Flk, and p-Flk in Group 3 was significantly lower than that in the control group after 7 days of treatment.

Conclusions: Topical itraconazole application was useful for effective inhibition of experimental corneal neovascularization.

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