June 2015
Volume 56, Issue 7
ARVO Annual Meeting Abstract  |   June 2015
Corneal Crosslinking with Ultraviolet-A and Riboflavin in a murine model of corneal neovascularisation
Author Affiliations & Notes
  • Felix Bock
    Department of Ophthalmology, University of Cologne, Cologne, Germany
  • Gabór Tóth
    Department of Clinical Ophthalmology, Semmelweis University, Budapest, Hungary
  • Nora Szentmary
    Klinik für Augenheilkunde, Universitätsklinikum des Saarlandes, Homburg/Saar, Germany
  • Franziska Bucher
    Department of Ophthalmology, University of Cologne, Cologne, Germany
  • Claus Cursiefen
    Department of Ophthalmology, University of Cologne, Cologne, Germany
  • Footnotes
    Commercial Relationships Felix Bock, None; Gabór Tóth, None; Nora Szentmary, None; Franziska Bucher, None; Claus Cursiefen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4517. doi:
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      Felix Bock, Gabór Tóth, Nora Szentmary, Franziska Bucher, Claus Cursiefen; Corneal Crosslinking with Ultraviolet-A and Riboflavin in a murine model of corneal neovascularisation. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4517.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: To analyse the impact of corneal crosslinking, using riboflavin and UVA-light illumination on existing blood and lymphatic vessels in the cornea.

Methods: We used the established murine model of suture-induced inflammatory corneal neovascularisation assay. 14 days after suture placement we removed the sutures.<br /> The mice underwent corneal abrasion and received cross-linking treatment using 0.1% riboflavin-5-phosphat solution (Mediocross H) (1x riboflavin-5-phosphat drop in 15 minutes) and subsequent UVA-light-illumination (370 nm; irradiance, 3 mW/cm2; dose, 5.4 J/cm2, 1x riboflavin-5-phosphat drop) for 15 minutes.<br /> After four days, corneal blood and lymphatic vessels were stained ex vivo using LYVE-1 and CD31 antibodies. Depth of cross linking was assessed by measuring the nucleus free area between Bowmann's layer and corneal endothelium using Dapi staining and an ApoTome2 microscope.

Results: The cross linked corneas showed an enhanced rigidity as a sign of successful cross linking and were not perforated. The corneal stroma was cross linked to a mean depth of 23.96% (SD 18.5%) of total corneal stromal thickness. Furthermore we found a significant negative correlation between the depth of cross linking and the area covered by blood vessels (p=0.018; r2= 0.881). Epithelial healing was delayed in the crosslinked animals.

Conclusions: We demonstrate a novel murine model of corneal crosslinkink. Crosslinking in murine corneas is a new way to analyse the effect of this clinically often used method on corneal cells. This pilot study shows that crosslinking can regress pathological corneal blood vessel in a depth dependent manner. Therefore this is a promising model to develop a new strategy to regress preexisting corneal vessels prior to keratoplasty.


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