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Wahiba Hadj Said, Diego García-Ayuso, Nicolas G Froger, Ivana Ivkovic, Manuel Jiménez-López, Julie Degardin, Manuel SIMONUTTI, Manuel Vidal-Sanz, Jose Alain Sahel, Serge A Picaud; Retinal distribution of cone and retinal ganglion cell degenerations in taurine-depleted mice. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4646.
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In 1970s, taurine deficiency was reported to induce photoreceptor degeneration. Surprisingly, we found that taurine depletion can also induce retinal ganglion cell (RGC) degeneration, which does not appear as a secondary degeneration because the degenerative process starts by the loss of both cone photoreceptors and RGCs (Gaucher et al.201, 2). Taurine can also prevent the degeneration of pure RGCs in culture (Froger et al., 2013) . To further investigate the relationship between cone and RGC losses, the distributions of RGCs and cones were quantified on flatmounted retina of taurine-depleted mice.
A group of albinos balb-c mice (n=10) was treated for 2 months with GES trough the drinking water (10g/L) whereas the control group of mice (n=10) drunk GES-free water. Electroretinogram (ERG) recordings were performed to verify the induction of a retinal damage retinal and retinal damages were examined in vivo, using optic coherence tomography (OCT). Retinal flatmounts were immunolabelled with Brn3a antibodies and L- and S-opsins to perform automatic RGC and cone quantification.
GES-treated mice showed a significant decrease of scotopoc a-wave ERG amplitudes and photopic ERG amplitudes, while amplitude of flickers were found significantly lower. OCT imaging displayed a significant reduction of the total retinal thickness in GES-treated mice when compared to untreated mice. Finally, in the automated counting confirmed the loss of Brn3a-positve RGCs and S- and L-opsins positive cone populations. Isodensity maps are under analysis to define more sensitive areas for RGC or cone degeneration.
These data confirm the vulnerability of RGCs, S- and L-cones to taurine depletion. The isodensity maps will be presented to define potential correlation in the degenerative processes for RGCs and cones. These data suggest that taurine depletion could play a role in retinal diseases similarly as its implication in the retinal toxicity of the antiepileptic drug, vigabatrin.
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