Purchase this article with an account.
Niyazi Acar, Olivier Berdeaux, Zhiguo HE, Stephanie Cabaret, Philippe Gain, Catherine P Garcher, Gilles Thuret, Alain M Bron, Lionel Bretillon; Associations between erythrocyte and ciliary epithelium phosphatidylcholine concentrations in healthy humans. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4668.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
The pathogenesis of primary open angle glaucoma (POAG) with elevated IOP lies in trabeculum. We have shown previously that red blood cell concentrations of several phosphatidylcholines, namely PC(18:0/22:6), PC(18:1/22:6), and PC(22:6/22:6), are reduced in patients with POAG (Acar et al Exp Eye Res 2009;89:840-853). As red blood cells lipid composition is considered as a good marker of the lipid profile of peripheral tissues, the aim of this work was to check whether the erythrocyte concentrations of PC(18:0/22:6), PC(18:1/22:6), and PC(22:6/22:6) are associated with that of phosphatidylcholines in the ciliary epithelium, the ocular structure responsible for aqueous humor production.
Red blood cells and ciliary epitheliums were collected from seven healthy human donors. Following lipid extraction, individual species of phosphatidylcholines were identified and quantified by HPLC-ESI-MS/MS on a triple quadrupole MS instrument. Spearman correlations were estimated for each compound to determine plausible associations between circulating and tissue concentrations.
Whereas the red blood cell levels of PC(18:0/22:6) and PC(22:6/22:6) did not show any relevant association with any phosphatidylcholine species in ciliary epithelium, the erythrocyte concentration of PC(18:1/22:6) was strongly and positively associated with that of PC(18:0/22:6) (rSpearman=0.828, p<0.05), PC(18:0/22:5) (rSpearman=0.882, p<0.05), PC(20:0/22:6) (rSpearman=0.882, p<0.05), PC(22:6/22:6) (rSpearman=0.936, p<0.05), and total phosphatidylcholines (rSpearman=0.954, p<0.05) in ciliary epithelium.
According to our data, PC(18:0/22:6) represents the best candidate for seeking correlations between peripheral blood and ciliary epithelium. Further studies in POAG globes are mandatory to confirm these observations.
This PDF is available to Subscribers Only