June 2015
Volume 56, Issue 7
ARVO Annual Meeting Abstract  |   June 2015
Control of lens fiber development by Lhx2-regulated neuroretinal Fgfs
Author Affiliations & Notes
  • Thuzar Thein
    Neuroscience, Johns Hopkins, Baltimore, MD
  • Jimmy de Melo
    Neuroscience, Johns Hopkins, Baltimore, MD
  • Cristina Zibetti
    Neuroscience, Johns Hopkins, Baltimore, MD
  • Seth Blackshaw
    Neuroscience, Johns Hopkins, Baltimore, MD
  • Footnotes
    Commercial Relationships Thuzar Thein, None; Jimmy de Melo, None; Cristina Zibetti, None; Seth Blackshaw, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 4835. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Thuzar Thein, Jimmy de Melo, Cristina Zibetti, Seth Blackshaw; Control of lens fiber development by Lhx2-regulated neuroretinal Fgfs. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4835. doi: https://doi.org/.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: Fibroblast growth factor (FGF) signaling is an essential regulator of lens epithelial cell proliferation and fiber cell differentiation. However, the identities of these Fgf factors, their source tissue, and the genes that regulate their synthesis are not well-characterized. In this study, we investigated the role of neuroretinal Lhx2 and Fgf factors in lens development.

Methods: Transgenic mice, Chx10-Cre and Pdgfrα-Cre, were used to delete Lhx2 and to overexpress Fgf10. Microarray, RNA-seq and qPCR analysis were conducted to investigate gene expression changes in these mice. ChIP-qPCR was performed to characterize Lhx2’s target genes. Immunohistochemistry and in situ hybridization were carried out to assess changes in morphology and molecular marker expression in these transgenic animals.

Results: We have found that Chx10-Cre; Lhx2F/F mice, which selectively lacked Lhx2 expression in neuroretina beginning at E10.5, showed an early arrest in lens fiber development along with severe microphthalmia and retinal disorganization. When Lhx2 was deleted in late retinal progenitors and Muller glial precursors using Pdgfrα-Cre, these mice developed early onset cataract and subsequent lens degeneration. Both mutants showed reduced expression of multiple neuroretinal-expressed FGFs in neuroretina, and likewise showed reduced expression of canonical FGF target genes in both the neuroretina and lens. When FGF expression was selectively restored in Lhx2-deficient neuroretina of Chx10-Cre; Lhx2F/F mice using a CAG-lox-stop-lox-Fgf10 transgene, we observed a dramatic rescue of the defects in lens but not in neuroretinal development.

Conclusions: Our data demonstrate that neuroretina-derived FGF factors are both necessary and sufficient to drive lens fiber development in vivo.


This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.