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Ji-Ae Ko, Chihiro Ohki, Akira Minamoto, Yosuke Sugimoto, Junko Hirata, Yoshiaki Kiuchi; Functional analysis of semaphorin 3F in rat retinal ganglion cells after optic nerve crush. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):4964.
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© ARVO (1962-2015); The Authors (2016-present)
We have previously shown that the expression of neural guidance protein, several groups of semaphorin in the retina. We have now examined the expression and function of semaphorin3F in retinal ganglion cells after optic nerve crush.
Optic nerve crush injury was induced in Long avans rats. Eyes and retinas were collected at intervals of 7 and 14 days after procedure. Using RT-PCR and immunoblot analysis, the expression levels of class 3 semaphorins were studied. Also, the expression and role of semaphorin3F in retinal ganglion cells after optic nerve crush was examined by Immunofluorescence analysis, Laser Microdissection and retinal organ culture system.
We found a decrease in semaphorin 3F levels in the retina at 7 and 14 days after optic nerve crush, but no change in others semaphorin 3 class (sema 3A, 3B or 3C). Particularly, these decreasing expression was seen in the retinal ganglion cell layers of retina. Furthermore, the down-regulation of RGCs marker, TJU-1 expression in RGC layer by knock-down expression system of semaphorin3F.
These results suggest that neural guidance gene semaphorin 3F may play an important role in the regulation of retinal ganglion cells functions and provide more information about RGC survival. It is indicated a useful factor from the viewpoint of neuroprotection.
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