Abstract
Purpose:
To explore the inhibitive effects of the nanoparticle-mediated delivery of plasminogen kringle 5(K5-NPs) on retinal scar formation induced by laser and to identify the potential pharmacologic mechanism targets.
Methods:
Retinal scar formation was induced by laser in 24 adult rats. Laser surgery was undergone on the retina and choroid of the right eye in rats and were divided into a control group and an experimental group (n=12/group). The medium with Control-NPs only or containing 10 μg/μL K5-NPs was injected in vitreous cavity. K5 expression was detected by immunohistochemistry. The expression of TGF-β, CTGF and α-SMA was detected by RT-PCR and Western blot analysis or Immunohistochemistry. retinal scar formation induced by laser and to identify the potential pharmacologic mechanism targets.
Results:
In comparison with the Control-NPs group, Intense K5 expression was detected in the retina on 14th day after the injection of K5-NPs. Histologic profiles showed that there was only a mild α-SMA in the retina in the K5-NPs group, but more severe α-SMA in the Control-NPs group. The expression of TGF-β and CTGF was downregulated by K5-NPs at the protein and mRNA levels.
Conclusions:
K5-NPs effectively inhibits laser induced retinal scar formation in rats. The mechanism may be that K5-NPs interferes in mRNA expression of TGF-β, thus abrogating TGF-β induced upregulation of CTGF,α-SMA.