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Ji Jin, Yi Lu, Weizhong Gu, Jianxing ma; Anti-fibrosis Effect of Nanoparticle-Mediated Delivery of a Natural Angiogenic Inhibitor. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):51.
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© ARVO (1962-2015); The Authors (2016-present)
To explore the inhibitive effects of the nanoparticle-mediated delivery of plasminogen kringle 5(K5-NPs) on retinal scar formation induced by laser and to identify the potential pharmacologic mechanism targets.
Retinal scar formation was induced by laser in 24 adult rats. Laser surgery was undergone on the retina and choroid of the right eye in rats and were divided into a control group and an experimental group (n=12/group). The medium with Control-NPs only or containing 10 μg/μL K5-NPs was injected in vitreous cavity. K5 expression was detected by immunohistochemistry. The expression of TGF-β, CTGF and α-SMA was detected by RT-PCR and Western blot analysis or Immunohistochemistry. retinal scar formation induced by laser and to identify the potential pharmacologic mechanism targets.
In comparison with the Control-NPs group, Intense K5 expression was detected in the retina on 14th day after the injection of K5-NPs. Histologic profiles showed that there was only a mild α-SMA in the retina in the K5-NPs group, but more severe α-SMA in the Control-NPs group. The expression of TGF-β and CTGF was downregulated by K5-NPs at the protein and mRNA levels.
K5-NPs effectively inhibits laser induced retinal scar formation in rats. The mechanism may be that K5-NPs interferes in mRNA expression of TGF-β, thus abrogating TGF-β induced upregulation of CTGF,α-SMA.
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