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Toco Yuen Ping Chui, Nikhil Menon, Nadim Choudhury, Alexander Pinhas, Michael Dubow, Nishit Shah, Alfredo Dubra, Richard B Rosen; Imaging of Periarteriolar Capillary Free Zone using Offset Pinhole Adaptive Optics Scanning Light Ophthalmoscopy. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5298.
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© ARVO (1962-2015); The Authors (2016-present)
Previous studies have shown that the dimension of the periarteriolar capillary free zone (CFZ) is associated with the retinal oxygen level. In this study, we imaged and quantified the CFZ in healthy controls using an offset pinhole adaptive optics scanning light ophthalmoscope (AOSLO).
Periarteriolar CFZ imaging was performed on 7 healthy controls (7 eyes, 6 males) using an offset pinhole AOSLO with an imaging wavelength centered at 790nm. Images were obtained along the retinal vascular arcades starting at the superior or inferior optic disc margin. Image sequences were acquired using a 1.5-degree field of view at a frame rate of 15Hz. After sinusoidal distortion and eye motion artifacts were removed, averaged vascular structural images and motion contrast vascular perfusion maps were obtained for quantitative measurements of lumen diameter, vessel wall thickness, and periarteriolar CFZ width using a semi-automatic MATLAB software.
Arteriolar lumen diameter, vessel wall thickness, and CFZ width were visualized in all subjects. CFZ was found at the same retinal depth as the arterioles. Vessels with a lumen diameters ranging in size from 16 to 76 µm were imaged and measured. The mean±SD µm of lumen diameter, vessel wall thickness, and CFZ was 42±15 µm, 10±2 µm, and 46±7 µm, respectively. The wall-to-lumen ratio was 0.24±0.08 µm (range: 0.13-0.44). The CFZ width measurements ranged from 36 to 71 µm. Although there was a weak positive correlation, no statistical significance was found between the CFZ width and lumen diameter (Linear Regression; p>0.05).
Offset pinhole AOSLO provides the ability to noninvasively and quantitatively assess periarteriolar CFZ. This has the potential for better understanding of the retinal vascular development in the healthy retina and in disease states such as diabetic retinopathy and retinopathy of prematurity.
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