Purchase this article with an account.
Shigeta Arichika, Akihito Uji, Nagahisa Yoshimura; Retinal arterial and venous wall visualization using nonconfocal split-detector adaptive optics laser scanning ophthalmoscopy. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5302.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
As it is well known that Adaptive Optics Scanning Laser Ophthalmoscopy (AOSLO) is a noninvasive technique enabling direct visualization of photoreceptors and vasculature, we decided to study the vascular wall structure of 3 normal subjects using our novel prototype of nonconfocal split-detector AOSLO system developed by Canon Inc.
In our split-detector nonconfocal AOSLO, confocal and 2 split nonconfocal signals were simultaneously recorded. The split-detector signal results from the difference between the intensities of the 2 nonconfocal signals divided by their sum at every pixel. The imaging light wavelength of our AOSLO was 840 nm and its optical resolution was 3 μm. The nonconfocal AOSLO images including split-detector images were acquired for 3 normal subjects (5 eyes; mean age, 35 years). The retinal area scanned was 1.4 × 1.4° and 2.8 × 2.8°, and AOSLO images of the upper largest temporal artery and vein were obtained. The scans were recorded for 3 s per area (frame rate, 32 Hz).
For all 3 subjects, artery and vein were clearly described.<br /> The average literal arterial and venous wall thickness was 10.8 µm and 4.91 µm for subject A, 8.96 µm and 5.13 µm for subject B, and 11.0 µm and 5.99 µm for subject C, respectively.
Nonconfocal split-detector AOSLO is a noninvasive technique which enables clear vascular visualization for both arteries and veins. This technique has the potential to identify preclinical or histological microvascular changes. However, further clinical studies are needed to validate our results.
This PDF is available to Subscribers Only