Purchase this article with an account.
Xiaoyan Chen, Jiao Wang, Lihua Wang, Dan-Ning Hu, Dongsheng Yan; MicroRNA-135b Inhibits Uveal Melanoma Cell Proliferation and Migration. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5317.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
MicroRNAs (miRNAs) can act as either oncogenes or tumor suppressors in tumorigenesis. Evidence indicates that miRNAs play important roles in uveal melanoma cell proliferation and migration. The role of miR-135b in uveal melanoma, however, remains unclear. Here, we investigated the function of miR-135b in uveal melanoma cells.
Realtime RT-PCR was performed to detect the expression of miR-135b in uveal melanoma specimens as well as normal controls. Transfection of miR-135b into uveal melanoma cells was carried out by Lipofectamine RNAiMAX reagent. The proliferation of uveal melanoma cells was measured by MTS assay. Cell cycle was analyzed by flow cytometry. Cell migration was examined by transwell migration assay.
miR-135b was decreased in uveal melanoma specimens as compared with normal controls. Transfection of miR-135b into uveal melanoma cells dramatically inhibited cell proliferation and blocked cell cycle at G1 phase. Furthermore, miR-135b suppressed cell migration.
Our results demonstrated that miR-135b may act as a tumor suppressor in uveal melanoma cell proliferation and migration.
This PDF is available to Subscribers Only