June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Expression Profiling of Keratoconic Cornea using MALDI-TOF mass spectrometry
Author Affiliations & Notes
  • HeeJung Ju
    Catholic Institutes of Visual Science, The Catholic University of Korea, Seoul, Korea (the Republic of)
  • Jeewon Mok
    Catholic Institutes of Visual Science, The Catholic University of Korea, Seoul, Korea (the Republic of)
  • Chang Rae Rho
    Catholic Institutes of Visual Science, The Catholic University of Korea, Seoul, Korea (the Republic of)
    Daejeon St. Mary's Hospital, Daejeon, Korea (the Republic of)
  • Ji Young Kwon
    Catholic Institutes of Visual Science, The Catholic University of Korea, Seoul, Korea (the Republic of)
  • Choun-Ki Joo
    Catholic Institutes of Visual Science, The Catholic University of Korea, Seoul, Korea (the Republic of)
    Seoul St. Mary’s hospital Eye Institute, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships HeeJung Ju, None; Jeewon Mok, None; Chang Rae Rho, None; Ji Young Kwon, None; Choun-Ki Joo, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5489. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      HeeJung Ju, Jeewon Mok, Chang Rae Rho, Ji Young Kwon, Choun-Ki Joo; Expression Profiling of Keratoconic Cornea using MALDI-TOF mass spectrometry. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5489.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To identify proteins differentially expressed between the cornea of keratoconus(KC) patient and control subject using two dimensional electrophoresis (2-DE) and mass spectrometry-based techniques

Methods: Proteins from the whole cornea were isolated from keratoconus and normal cornea tissues, and separated by two-dimensional gel electrophoresis (2-DE). Comparison of protein patterns was performed using PDQuest Software and protein differences were identified 100 differential protein spots by mass spectrometry. All of the 100 protein spots were analyzed the peptide mass finger printing (PMF) with the 4700 MALDI-TOF/TOF mass spectrometer to deduce their possible functions. Finally, results were validated by western-blot

Results: Of the 100 protein spots, we identified 51 spots that demonstrated a significant differences between keratoconus and reference normal cornea with a ProtScore (>or=2). Particularly, the intensity of 37 spots were markedly higher in keratoconus than normal cornea (>2 folds), and 14 spots were detected only in keratoconic cornea. Among them, transforming growth factor-beta-induced protein ig-h3 precursor, Keratin 14, and alpha-crystallin B chain were more expressed in keratoconus pateints and aldehyde dehydrogenase 1, keratin 3, alpha-enolase and peroxiredoxin-6 were more expressed in control

Conclusions: In this study, we have presented the finding of proteins differentially expressed in the keratoconus patients. The identified proteins corresponded to molecules belonging to ECM related cytoskeleton, antioxidant protein and transcription/transformation. Our findings could contribute to the knowledge for of pathogenesis of keratoconus

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×