June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Experimental optic neuritis induced by the microinjection of lipopolysaccharide into the optic nerve
Author Affiliations & Notes
  • Marcos Luis Aranda
    Human Biochemistry, University of Buenos Aires /Sch of Medicine, Buenos Aires, Argentina
  • Damian Dorfman
    Human Biochemistry, University of Buenos Aires /Sch of Medicine, Buenos Aires, Argentina
  • Pablo Sande
    Human Biochemistry, University of Buenos Aires /Sch of Medicine, Buenos Aires, Argentina
  • Ruth Estela Rosenstein
    Human Biochemistry, University of Buenos Aires /Sch of Medicine, Buenos Aires, Argentina
  • Footnotes
    Commercial Relationships Marcos Luis Aranda, None; Damian Dorfman, None; Pablo Sande, None; Ruth Rosenstein, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5529. doi:https://doi.org/
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      Marcos Luis Aranda, Damian Dorfman, Pablo Sande, Ruth Estela Rosenstein; Experimental optic neuritis induced by the microinjection of lipopolysaccharide into the optic nerve. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5529. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Optic neuritis (ON) is a condition involving primary inflammation, demyelination, and axonal injury in the optic nerve which leads to retinal ganglion cell (RGC) loss, and visual dysfunction. We investigated the ability of a single microinjection of bacterial lipopolysaccharide (LPS) directly into the optic nerve to induce functional and structural alterations compatible with ON. For this purpose, optic nerves from male Wistar rats remained intact or were injected with vehicle or LPS.

Methods: The effect of LPS was evaluated at several time points post-injection in terms of: i) visual pathway and retinal function (visual evoked potentials (VEPs) and electroretinograms, (ERGs), respectively), ii) anterograde transport from the retina to its projection areas, iii) consensual pupil light reflex (PLR), iv) optic nerve histology, v) microglia/macrophage reactivity (by Iba-1- and ED1-immunostaining), vi) astrocyte reactivity (by glial fibrillary acid protein-immunostaining), vii) axon number (by toluidine blue staining), vii) demyelination (by myelin basic protein immunoreactivity and luxol fast blue staining), viii) optic nerve ultrastructure, and ix) RGC number (by Brn3a immunoreactivity).

Results: LPS induced a significant and persistent decrease in VEP amplitude and PLR,without changes in the ERG. In addition, LPS induced a deficit in anterograde transport, and an early inflammatory response consisting in an increased cellularity, and Iba-1 and ED1-immunoreactivity in the optic nerve, which were followed by changes in axonal density, astrocytosis, demyelination, and axon and RGC loss.

Conclusions: These results suggest that the microinjection of LPS into the optic nerve may serve as a new experimental model of primary ON.

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