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Keith P Van Nostrand, David Barnard, Angel Payan, Alexis Russell, Kirsten J Lampi, Lea V Michel, George M Thurston; Static and quasielastic light scattering studies of beta crystallin solutions. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5592.
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© ARVO (1962-2015); The Authors (2016-present)
To study the development of high concentration short-range order in beta crystallin solutions, and accompanying protein dynamics.
Bovine beta crystallin fractions were isolated using size-exclusion chromatography of 1-2 week old calf lens homogenates, and studied using static and quasielastic light scattering of 633 nm wavelength light, at concentrations up to 250 milligrams/milliter (mg/ml). These measurements yield excess Rayleigh ratios, or absolute cross sections for light scattering, and corresponding collective diffusion coefficients.
For higher molecular weight fractions averaging 7x104 grams/mole, as determined from dilute concentration Rayleigh ratios, high concentration Rayleigh ratios plateau in the vicinity of 200 to 250 mg/ml, at close to 1.5 x 10-3 inverse centimeters, while low concentration scattering is intermediate between those of alpha and gamma crystallin solutions. Diffusion coefficients decrease in a nonlinear fashion from 5.5 x 10-11 m2/sec to 4 x 10-11 m2/sec over the same concentration range.
The observed dependence of the Rayleigh ratios on concentration is consistent with the influence of repulsive interactions between beta crystallins, as reported previously, which can lead to short-range order at high concentrations.
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