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Yangluowa Qu, Tingting Liu, Dulei Zou, Changkai Jia, Liying Zhang, Sanming Li, Zuguo Liu, Wei Li; Brg1 is associated with corneal epithelial development and differentiation. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5642.
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© ARVO (1962-2015); The Authors (2016-present)
Brahma-related gene 1 (Brg1) is the catalytic ATPase subunit of SWI/SNF that is one of five chromatin remodeling complexes exist in eukaryotic cells. Previous studies showed Brg1 binding complex is involved in cell cycle control, apoptosis and cell differentiation in different cell types, while there is no report about the function of Brg1 in the limbal and corneal tissues.
Human and rabbit corneal limbal tissues were used for cryosection or epithelial cell culture. The human corneal epithelial cell line (HCE), rabbit and mouse primary corneal epithelial cells were cultured in DMEM/F12+10%FBS, SHEM and Cn-20 medium, respectively. Rabbit limbal epithelial cells were seeded on 3T3 feeder layers for clonal culture. The expression of Brg1 on mouse corneas from postnatal day to 8 weeks was evaluated. The brg1 protein production and localization were determined by immunofluorescent staining and Western blot analysis.
Brg1 was absent in the basal layer of the human limbal epithelium, while was expressed in the superficial layer of limbal and peripheral corneal epithelium and full-thickness of the central corneal epithelial cells. The similar expression pattern of Brg1 was found in rabbit limbal and corneal tissues. However, Brg1 was not detected in human skin and conjunctival tissues. Brg1 nuclear expression was found in HCE cell line and primary cultured rabbit corneal epithelial cells. Brg1 showed negative expression in rabbit limbal epithelial clone cultures. Double-staining showed that Brg1 was co-localized with Pax6 in primary mouse corneal epithelial cells. Brg1 was negative in P0 mouse corneal epithelium, while commenced at P3, gradually increased from 2 to 3 weeks postnatally, and showed full thickness expression in the central corneal epithelium on 4 and 8 weeks.
Brg1 expression is associated with corneal epithelial lineage commitment, maturation and differentiation.
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