June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Hydrogel sustained-release of C3 exoenzyme lowered IOP in ocular hypertensive rats
Author Affiliations & Notes
  • Xuyang Liu
    Shenzhen Eye Hospital, Shenzhen, China
  • Fen He
    Sichuan University, Chengdu, China
  • Yun Wang
    Shenzhen Eye Hospital, Shenzhen, China
  • ning fan
    Shenzhen Eye Hospital, Shenzhen, China
  • Iok-Hou Pang
    University of North Texas Health Science Center, Denton, TX
  • Footnotes
    Commercial Relationships Xuyang Liu, None; Fen He, None; Yun Wang, None; ning fan, None; Iok-Hou Pang, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 5689. doi:https://doi.org/
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      Xuyang Liu, Fen He, Yun Wang, ning fan, Iok-Hou Pang; Hydrogel sustained-release of C3 exoenzyme lowered IOP in ocular hypertensive rats. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5689. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To evaluate the IOP-lowering effect of hydrogel sustained-release of C3 exoenzyme in rat eyes.

Methods: C3 exoenzyme was expressed in E.coli and purified by affinity chromatography. Immunofluorescence was performed in NIH 3T3 cells treated with C3 to verify the cellular uptake of the protein. A sustained-release formulation was prepared comprising a bio-absorbable polymer hydrogel and C3 transferase. IOP of Sprague-Dawley rats was measured with the TonoLab rebound tonometer. Baseline IOP was obtained before an intracameral injection of 6 µl hydrogel (30%) into both eyes to induce an acute ocular hypertension. IOP was measured at 2, 14 and 24 h post-injection. After 24 h, 6 µl of the sustained-release formulation (30% hydrogel containing 15 µg C3 exoenzyme) was injected into the anterior chamber of the right eyes, while 6 µl hydrogel (30%) was injected into the anterior chamber of left eyes as control. IOP was measured at 2 h and then every 12 h following the second intracameral injection.

Results: Intracameral injection of hydrogel raised rat IOP to 35~47 mmHg. The sustained-release of C3 exoenzyme significantly lowered IOP. Its maximal IOP reduction effect was 41% (17.2 ± 2.2 mmHg) at 26 h after the second injection (p < 0.05). The IOP-lowering effect of C3 exoenzyme lasted about 60 h.

Conclusions: Hydrogel sustained-release of C3 exoenzyme can reduce IOP in a rat model with ocular hypertension.

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