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Xuyang Liu, Fen He, Yun Wang, ning fan, Iok-Hou Pang; Hydrogel sustained-release of C3 exoenzyme lowered IOP in ocular hypertensive rats. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5689. doi: https://doi.org/.
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To evaluate the IOP-lowering effect of hydrogel sustained-release of C3 exoenzyme in rat eyes.
C3 exoenzyme was expressed in E.coli and purified by affinity chromatography. Immunofluorescence was performed in NIH 3T3 cells treated with C3 to verify the cellular uptake of the protein. A sustained-release formulation was prepared comprising a bio-absorbable polymer hydrogel and C3 transferase. IOP of Sprague-Dawley rats was measured with the TonoLab rebound tonometer. Baseline IOP was obtained before an intracameral injection of 6 µl hydrogel (30%) into both eyes to induce an acute ocular hypertension. IOP was measured at 2, 14 and 24 h post-injection. After 24 h, 6 µl of the sustained-release formulation (30% hydrogel containing 15 µg C3 exoenzyme) was injected into the anterior chamber of the right eyes, while 6 µl hydrogel (30%) was injected into the anterior chamber of left eyes as control. IOP was measured at 2 h and then every 12 h following the second intracameral injection.
Intracameral injection of hydrogel raised rat IOP to 35~47 mmHg. The sustained-release of C3 exoenzyme significantly lowered IOP. Its maximal IOP reduction effect was 41% (17.2 ± 2.2 mmHg) at 26 h after the second injection (p < 0.05). The IOP-lowering effect of C3 exoenzyme lasted about 60 h.
Hydrogel sustained-release of C3 exoenzyme can reduce IOP in a rat model with ocular hypertension.
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