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Sebastian Mueller, Carlo Krupp, Sven Schnichels, Johanna Hofmann, Martin Stephan Spitzer, Karl Ulrich Bartz-Schmidt, Peter Szurman, Kai Januschowski; Investigating Retinal Toxicity of a Lutein-Based Dye in a Model of Isolated and Perfused Bovine Retina. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):5737.
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© ARVO (1962-2015); The Authors (2016-present)
Retidyne™ is a new lutein-based dye for internal limiting membrane staining. It uses the intrinsic staining characteristics of lutein which is already known to act as an antioxidant in the human retina. We investigated retinal tolerance to different staining times measured by the electroretinogram (ERG) of an isolated and perfused retina whole mount.
For functionality testing bovine retinas were prepared and perfused with an oxygen saturated standard solution and the ERG was recorded until stable b-wave amplitudes were reached. Then the perfusion was stopped and Retidyne™ was applied directly onto the retinal surface for exposure times of 60 or 120 seconds. After restarting the perfusion with standard solution, the ERG amplitudes were monitored for 70 minutes. To investigate the effects on photoreceptor function alone, 1 mM asparate was added to block b-waves.
For an exposure time of 60 seconds amplitudes of a- and b-waves remained stable throughout the experiment. Exposure times of 120 seconds caused an initial drop of amplitudes which reached statistical significance only for a-waves (a: -21%, p=0.047;<br /> b: -14%, p=0.052). This effect was only seen during the first minutes of the wash-out and the ERG recovered completely.
In the model of isolated and perfused bovine retina Retidyne™ showed a good safety profile for common intraoperatively used staining times. The initial drop of amplitudes when exposing the retina to a longer time than usually used may be related to a lack of oxygen or nutrients as well as blockage of the flashlight due to a more intense staining effect at the beginning of the wash-out.
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