June 2015
Volume 56, Issue 7
Jump To...
Free
ARVO Annual Meeting Abstract  |   June 2015
Test-Retest Reliability of Hemifield, Central-field and Full-field Chromatic Pupillometry For Assessing the Function of Melanopsin-containing Retinal Ganglion Cells
Shaobo Lei; Herbert C. Goltz; Manokaraananthan Chandrakumar; Agnes MF Wong
Author Affiliations & Notes
  • Shaobo Lei
    Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, ON, Canada
  • Herbert C. Goltz
    Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada
    Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, ON, Canada
  • Manokaraananthan Chandrakumar
    Ophthalmology and Vision Sciences, The Hospital for Sick Children, Toronto, ON, Canada
  • Agnes MF Wong
    Ophthalmology and Vision Sciences, The Hospital for Sick Children, Toronto, ON, Canada
    Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada
  • Footnotes
    Commercial Relationships Shaobo Lei, None; Herbert Goltz, None; Manokaraananthan Chandrakumar, None; Agnes Wong, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 577. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Test-Retest Reliability of Hemifield, Central-field and Full-field Chromatic Pupillometry For Assessing the Function of Melanopsin-containing Retinal Ganglion Cells
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      Shaobo Lei, Herbert C. Goltz, Manokaraananthan Chandrakumar, Agnes MF Wong; Test-Retest Reliability of Hemifield, Central-field and Full-field Chromatic Pupillometry For Assessing the Function of Melanopsin-containing Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):577.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract
 
Purpose
 

The post-illumination pupil response (PIPR) is an index of melanopsin-driven intrinsically photosensitive retinal ganglion cell (ipRGC) activity. Hemifield analysis of PIPR may have important clinical implications. We developed a novel method to induce PIPR during hemifield stimulation and evaluated the test-retest reliability of current methods of measuring PIPR under hemifield, central-field and full-field stimulation.

 
Methods
 

Pupil response was recorded with an eye tracker in 10 visually normal subjects (6 females, mean age 30 years, range 19-56). Light stimuli were presented using a Ganzfeld screen with a custom built device to control the extent of retina being stimulated. Blue light stimulation at 400 cd/m2 intensity was presented for 400 ms to the lower and upper halves of the central 30° (hemifields), central 30° (central-field) and full-field to induce PIPR. Red full-field stimulations at the same intensity and duration were also presented as an internal control condition. Test-retest reliability of the PIPR measures were assessed by calculating the intra-class correlation coefficient (ICC) of 6 repetitions for lower and upper hemifield stimulation, and 3 repetitions for central-field and full-full stimulation.

 
Results
 

Hemifield, central-field and full-field blue light stimulation induced increasingly greater PIPR in ascending order , while full-field red light stimulation induced no PIPR. Mean lower and upper hemifield PIPR were highly symmetric (Figure 1). Mean ICC of blue light PIPR was 0.87 for lower/upper hemifield, 0.88 for central-field, and 0.94 for full-field stimulation.

 
Conclusions
 

We described a convenient yet reliable method to measure PIPR induced by hemifield, central-field and full-field light stimulation. Good PIPR measurement reliability was obtained under all viewing area conditions. This protocol will facilitate the clinical applications of PIPR testing.  

Figure 1: Mean pupil responses from 10 visually-normal subjects. Pupil diameter data were normalized to baseline recording for 5 s prior to the onset of blue or red light stimulation (400ms, 400cd/m2). LHF, Lower Hemifield; UHF, Upper Hemifield; CF, Central-field; and FF, full-field.
View OriginalDownload Slide
 
Figure 1: Mean pupil responses from 10 visually-normal subjects. Pupil diameter data were normalized to baseline recording for 5 s prior to the onset of blue or red light stimulation (400ms, 400cd/m2). LHF, Lower Hemifield; UHF, Upper Hemifield; CF, Central-field; and FF, full-field.
 
Figure 1: Mean pupil responses from 10 visually-normal subjects. Pupil diameter data were normalized to baseline recording for 5 s prior to the onset of blue or red light stimulation (400ms, 400cd/m2). LHF, Lower Hemifield; UHF, Upper Hemifield; CF, Central-field; and FF, full-field.
Figure 1: Mean pupil responses from 10 visually-normal subjects. Pupil diameter data were normalized to baseline recording for 5 s prior to the onset of blue or red light stimulation (400ms, 400cd/m2). LHF, Lower Hemifield; UHF, Upper Hemifield; CF, Central-field; and FF, full-field.
View OriginalDownload Slide

 
The Association for Research in Vision and Ophthalmology
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept