June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Test-Retest Reliability of Hemifield, Central-field and Full-field Chromatic Pupillometry For Assessing the Function of Melanopsin-containing Retinal Ganglion Cells
Author Affiliations & Notes
  • Shaobo Lei
    Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, ON, Canada
  • Herbert C. Goltz
    Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada
    Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, ON, Canada
  • Manokaraananthan Chandrakumar
    Ophthalmology and Vision Sciences, The Hospital for Sick Children, Toronto, ON, Canada
  • Agnes MF Wong
    Ophthalmology and Vision Sciences, The Hospital for Sick Children, Toronto, ON, Canada
    Ophthalmology and Vision Sciences, University of Toronto, Toronto, ON, Canada
  • Footnotes
    Commercial Relationships Shaobo Lei, None; Herbert Goltz, None; Manokaraananthan Chandrakumar, None; Agnes Wong, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 577. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Shaobo Lei, Herbert C. Goltz, Manokaraananthan Chandrakumar, Agnes MF Wong; Test-Retest Reliability of Hemifield, Central-field and Full-field Chromatic Pupillometry For Assessing the Function of Melanopsin-containing Retinal Ganglion Cells. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):577. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

The post-illumination pupil response (PIPR) is an index of melanopsin-driven intrinsically photosensitive retinal ganglion cell (ipRGC) activity. Hemifield analysis of PIPR may have important clinical implications. We developed a novel method to induce PIPR during hemifield stimulation and evaluated the test-retest reliability of current methods of measuring PIPR under hemifield, central-field and full-field stimulation.

 
Methods
 

Pupil response was recorded with an eye tracker in 10 visually normal subjects (6 females, mean age 30 years, range 19-56). Light stimuli were presented using a Ganzfeld screen with a custom built device to control the extent of retina being stimulated. Blue light stimulation at 400 cd/m2 intensity was presented for 400 ms to the lower and upper halves of the central 30° (hemifields), central 30° (central-field) and full-field to induce PIPR. Red full-field stimulations at the same intensity and duration were also presented as an internal control condition. Test-retest reliability of the PIPR measures were assessed by calculating the intra-class correlation coefficient (ICC) of 6 repetitions for lower and upper hemifield stimulation, and 3 repetitions for central-field and full-full stimulation.

 
Results
 

Hemifield, central-field and full-field blue light stimulation induced increasingly greater PIPR in ascending order , while full-field red light stimulation induced no PIPR. Mean lower and upper hemifield PIPR were highly symmetric (Figure 1). Mean ICC of blue light PIPR was 0.87 for lower/upper hemifield, 0.88 for central-field, and 0.94 for full-field stimulation.

 
Conclusions
 

We described a convenient yet reliable method to measure PIPR induced by hemifield, central-field and full-field light stimulation. Good PIPR measurement reliability was obtained under all viewing area conditions. This protocol will facilitate the clinical applications of PIPR testing.  

 
Figure 1: Mean pupil responses from 10 visually-normal subjects. Pupil diameter data were normalized to baseline recording for 5 s prior to the onset of blue or red light stimulation (400ms, 400cd/m2). LHF, Lower Hemifield; UHF, Upper Hemifield; CF, Central-field; and FF, full-field.
 
Figure 1: Mean pupil responses from 10 visually-normal subjects. Pupil diameter data were normalized to baseline recording for 5 s prior to the onset of blue or red light stimulation (400ms, 400cd/m2). LHF, Lower Hemifield; UHF, Upper Hemifield; CF, Central-field; and FF, full-field.

 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×