Purchase this article with an account.
Thomas Ritter, Grace A. O'Malley, Paul Lohan, Aideen Ryan, Matthew Griffin, Sweta Rani; Mesenchymal stem cell-derived extracellular vesicles promote corneal wound repair. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):716.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate if topically applied mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) promote corneal wound repair.
EVs were isolated from media conditioned by human bone marrow-derived MSCs (n=4) using series of centrifugation, filtration (0.22μm) and ultracentrifugation. Transmission electron microscopy (TEM) was used to detect classical protein markers and size of EVs were determined using nanoparticle tracking analysis (NTA; NanoSight). Pro-healing effects of MSC-derived EV were examined in vitro by cell migration and scratch-wound-healing assays using human corneal epithelial (HCE) and endothelial (HCEC) cell lines. Moreover, angiogenesis was also studied using HUVEC cells on matrigel coating.
Successful isolation of EVs was confirmed by immune-gold TEM showing expression of classical exosomal markers CD63 and TSG101 on their surface. NTA showed the average size of EVs ranged between 50-130nm. Significant increases in HCE and HCEC cell migration in both cell lines studied in the presence of MSC-EVs (<45%=HCEC; <93%=HCE) could be observed compared to PBS and EV-depleted media conditioned (dCM) by MSCs. Similarly, a significant increase in scratch-wound closure of the cells treated with EV was observed compared to PBS (<75%=HCEC; <80%=HCE) or dCM-treated cells. Interestingly, we also found increased capacity of angiogenesis in HUVEC cells when treated with MSC-derived EVs compared to PBS or dCM.
This study supports that MSC-derived EV show promising therapeutic potential and could be considered as an option for cell-free treatment in ocular surface disease.
This PDF is available to Subscribers Only