Purchase this article with an account.
Sri Magadi, Zhijie Li, Wanyu Zhang, Debjani Phillips, Rolando Rumbaut, Clifton Wayne Smith, Alan Robert Burns; Platelet Recruitment is Associated with Limbal Venule Expansion in a Mouse Model of Corneal Abrasion. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):743.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
A hallmark of acute inflammation is vasodilation. The extravasation of neutrophils and platelets after corneal abrasion is beneficial to wound healing. In mast cell deficient mice (Kitw-sh/w-sh), limbal venule expansion is blunted, there is a marked delay in neutrophil extravasation, and little or no platelet extravasation. The purpose of this study was to examine the relationship between venule expansion and platelet extravasation.
Adult C57BL/6 mice were anesthetized and a 2mm central corneal abrasion was made using a golf-club spud or Alger brush. Prior to injury, one group of wild type mice was treated with anti-Ly6G antibody to deplete neutrophils which in turn limits platelet recruitment. A second group had reduced leukocyte CD18 expression (CD18 mutant) with normal neutrophil extravasation but limited platelet recruitment. The third group of wild type injured mice received topical treatment with rIL-20 (200 ng/ml) once every 4h for 24h which inhibits neutrophil and platelet extravasation. A control group of injured wild type mice received phosphate buffered saline topically. At 24h post-injury, excised corneas were immunostained for vessels and platelets. Limbal venule diameters were evaluated and analyzed using the Kolmogorov-Smirnov test.
Prior to injury, limbal venule diameters were not different across mouse groups. At 24h post-injury, in wild type mice, the average limbal venule diameters increased from 15 μm to 25 μm (p<0.05 compared to uninjured baseline). Injured CD18 mutant mice and mice treated with anti-Ly6G or rIL-20 showed a marked reduction (>70%) in platelet extravasation compared to injured wild type mice. The average venule diameters in injured CD18 mutant mice and mice treated with anti-Ly6G or rIL-20 were similar across the groups, increasing to ~20 μm which was significantly less than that observed in injured wild type mice.
In three experimental models that significantly reduce platelet extravasation following corneal injury, limbal venule expansion was significantly reduced, suggesting possible functional links between venule expansion and platelet extravasation.
This PDF is available to Subscribers Only