June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Bone marrow-derived cells contribute to choroidal neovascularization by MMPs expression regulated by miRNA
Author Affiliations & Notes
  • Huiyuan Hou
    Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi’an, China
  • Yang Lv
    Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi’an, China
  • Fan Gao
    Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi’an, China
  • Hongjun Du
    Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi’an, China
  • Yusheng Wang
    Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi’an, China
  • Footnotes
    Commercial Relationships Huiyuan Hou, None; Yang Lv, None; Fan Gao, None; Hongjun Du, None; Yusheng Wang, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 806. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Huiyuan Hou, Yang Lv, Fan Gao, Hongjun Du, Yusheng Wang; Bone marrow-derived cells contribute to choroidal neovascularization by MMPs expression regulated by miRNA. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):806.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

To prove whether bone marrow-derived cells(BMCs), which are involved in the development of choroidal neovascularization (CNV) , are the main source of matrix metalloproteinases (MMPs) , and whether the MMP-2/-13 expression is potentially regulated by miR188-5p

 
Methods
 

BMCs of GFP transgenic mice were transplanted to wild-type C57 mice to establish chimeras, and CNV was induced by laser. MMP-2/MMP-13 levels in hypoxia-treated bone marrow-derived mesenchymal stem cells (BMSCs) and in CNV eyes were quantified by western blot. miR-188-5p levels were detected using qRT-PCR. Immunofluorescence staining (IF) was used to identify MMP-2/MMP-13 and miR188-5p expressed by GFP -positive BMCs or cells in situ in CNV, and the expression levels were semi-quantified by images analysis.

 
Results
 

In vitro, expression of miR-188-5p decreased, while the mRNA and protein levels of MMP-2/13 significantly increased in hypoxia-treated BMSCs (p < 0.05). In vivo, western blot and IF showed MMP-2 expression rapidly increased after CNV induction and reached the climax on day 3, when the corresponding expression in BMCs reached the peak (64.21% of the total expression in CNV). MMP-13 expression showed relative slow increase at the early stage of CNV and reached the climax on day 7, meanwhile the expression in BMCs also reached the highest 79.61% of the total expression. qRT-PCR and IF results demonstrated that miR188-5p expression fell rapidly after laser, and the lowest expression level was observed on day 7. MMP-2 / MMP-13 protein expression in CNV was negatively correlated with miR188-5p expression. Similarly, IF semi-quantitative analysis of the expression of MMP-13 in BMCs in CNV showed a negative correlation with the expression of miR188-5p in BMCs (r=-0.868,p<0.05). While the expression of MMP-2 in BMCs showed a negative correlation with miR188-5p only in the first 5 days after CNV induction (r=-0.997,p<0.05)(Figure). The predict result of target genes showed that 3’UTRs of MMP-2 and MMP-13 have complementary association points of miR188-5p.

 
Conclusions
 

In experimental CNV, MMPs partially expressed by BMCs. BMCs may play the main role of regulating MMPs expression in CNV, and the expression of MMP-2/MMP-13 from BMCs might be regulated by miR188-5p. Further investigation is needed to confirm the regulation of MMPs in BMCs by miRNA.  

 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×