March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Femtosecond Assisted Technique In The Donor Harvesting For Endothelial Keratoplasty
Author Affiliations & Notes
  • Juan A. Duran
    Instituto de Oftalmologia, Basque Country University, Vizcaya, Spain
  • Arturo E. Grau
    Instituto de Oftalmologia, Moorfields Eye Hospital, London, United Kingdom
  • Mikel García
    Instituto de Oftalmologia, ICQO, Vizcaya, Spain
  • Footnotes
    Commercial Relationships  Juan A. Duran, None; Arturo E. Grau, None; Mikel García, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 34. doi:
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      Juan A. Duran, Arturo E. Grau, Mikel García; Femtosecond Assisted Technique In The Donor Harvesting For Endothelial Keratoplasty. Invest. Ophthalmol. Vis. Sci. 2012;53(14):34. doi:

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To describe a new harvesting technique to obtain a corneal graft for endothelial keratoplasty.

Methods: : The donor cornea is placed in an artificial anterior chamber and its apex is marked with a pen. After pachymetry at the 8-9 mm corneal diameter, the femtolaser (IntraLase™) suction ring is docked to the applanation cone. Ring tunneling is performed with the following laser settings: outer diameter 9.0 mm; inner diameter 7.7 mm; depth 450-500 µm; ring energy 170 mJ. A custom-designed hook (outer diameter 8.3 mm; inner diameter 6.8 mm; a small hole near the tip, and an arch length of 320º) which has been threaded with a 6/0 nylon suture is then passed through the tunnel. Corneal delamination is achieved by applying traction to the suture. The delamination is enlarged towards the periphery with a spatula.Once the cornea was punched, the anterior interface was studied under SEM, to analize the quality of the interface.

Results: : We have studied the characteristics of the buttons in 12 eyes from 12 patients. In all cases, a homogeneous thin graft was obtained, without perforation or loss of the lamellar plane.

Conclusions: : With this technique, we can reliably obtain a graft for endothelial keratoplasty, with predictable thickness and shape characteristics.

Keywords: transplantation • cornea: endothelium • cornea: clinical science 

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