March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Expression of Angiogenesis-related Factors in Human Corneas after Cultivated Oral Mucosal Epithelial Transplantation
Author Affiliations & Notes
  • Hung-Chi J. Chen
    Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
  • Lung-Kun Yeh
    Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
  • Chi-Chin Sun
    Ophthalmology, Chang Gung Memorial Hospital, Keelung, Taiwan
  • Scheffer C. Tseng
    Ocular Surface Center, Ocular Surface Res & Educ Fndtn, Miami, Florida
  • Jan-Kan Chen
    Physiology, Chang Gung University, Taoyuan, Taiwan
  • David H. Ma
    Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
  • Footnotes
    Commercial Relationships  Hung-Chi J. Chen, None; Lung-Kun Yeh, None; Chi-Chin Sun, None; Scheffer C. Tseng, None; Jan-Kan Chen, None; David H. Ma, None
  • Footnotes
    Support  National Science Council, Taiwan
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 269. doi:
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      Hung-Chi J. Chen, Lung-Kun Yeh, Chi-Chin Sun, Scheffer C. Tseng, Jan-Kan Chen, David H. Ma; Expression of Angiogenesis-related Factors in Human Corneas after Cultivated Oral Mucosal Epithelial Transplantation. Invest. Ophthalmol. Vis. Sci. 2012;53(14):269.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To analyze the expression of angiogenesis-related factors in corneal tissues previously undergoing autologous cultivated oral mucosal epithelial transplantation (COMET).

Methods: : Six eyes from four chemically and two thermally injured patients with limbal stem cell deficiency who received COMET to promote wound healing were retrospectively studied. Immunoconfocal microscopy was performed on corneal specimens from above-mentioned patients after COMET as well as normal cornea and oral mucosa for keratin 8 (K-8), fibroblast growth factor-2 (FGF-2), vascular endothelial growth factor (VEGF), collagen XVIII (endostatin), pigment epithelium-derived factor (PEDF), soluble fms-like tyrosine kinase-1 (sFlt-1), tissue inhibitor of metalloprotease-3 (TIMP-3), thrombospondin-1 (TSP-1), and interleukin-1 receptor antagonist (IL-1ra).

Results: : FGF-2, VEGF, endostatin, PEDF, and IL-1ra were universally detected in all samples, among which signal for FGF-2, VEGF and IL-1ra was localized to full-thickness epithelial layer, while endostatin was limited to the basement membrane. Expression of PEDF varied in tissues from all six patients, with a preferential expression in the suprabasal epithelial layer. FGF-2 and IL-1ra were over-expressed in basal epithelial layer in specimens with increased stratification. Signal for sFlt-1, TIMP-3, and TSP-1 was positive only in normal cornea and corneal epithelium in the specimen from Patient 3, while it was negative in all other specimens.

Conclusions: : Immunoconfocal expression of FGF-2, VEGF, PEDF, endostatin, and IL-1ra was similar in normal cornea, oral mucosa, and corneas after COMET. Expression of sFlt-1, TIMP-3, and TSP-1 was negligible in oral mucosal epithelial cells and corneas after COMET. Lack of above-mentioned anti-angiogenic factors may contribute to peripheral corneal neovascularization often seen after COMET.

Keywords: cornea: clinical science • transplantation • immunohistochemistry 

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