March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Polyesteramide Microparticles For Ophthalmic Drug Delivery
Author Affiliations & Notes
  • Vanessa Andres-Guerrero
    Pharmaceutical Technology,
    Faculty of Pharmacy/Complutense University, Madrid, Spain
  • Beatriz de las Heras
    Faculty of Pharmacy/Complutense University, Madrid, Spain
  • George Mihov
    DSM Ahead & DSM Biomedical, Geleen, The Netherlands
  • Aylvin Dias
    DSM Ahead & DSM Biomedical, Geleen, The Netherlands
  • Rocío Herrero-Vanrell
    Pharmaceutical Technology,
    Faculty of Pharmacy/Complutense University, Madrid, Spain
  • Footnotes
    Commercial Relationships  Vanessa Andres-Guerrero, None; Beatriz de las Heras, None; George Mihov, DSM Biomedical (E); Aylvin Dias, DSM Biomedical (E); Rocío Herrero-Vanrell, None
  • Footnotes
    Support  PANOPTES (project number 246180) under the 7th Research Framework Programme of the European Union and Spanish Ministry of Health (RETICS RD07/0062).
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 476. doi:
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      Vanessa Andres-Guerrero, Beatriz de las Heras, George Mihov, Aylvin Dias, Rocío Herrero-Vanrell; Polyesteramide Microparticles For Ophthalmic Drug Delivery. Invest. Ophthalmol. Vis. Sci. 2012;53(14):476.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Polyesteramides (PEAs) are a new family of polymeric materials. PEAs combine good mechanical, thermal and processing properties and are also biodegradable. The purpose of the current study was to evaluate PEA-II microparticles to be used as carriers for controlled drug delivery in the eye.

Methods: : Microparticles (MPs) were prepared using an emulsion-solvent evaporation technique. Particle size and morphology of MPs were characterized by dynamic light scattering and scanning electron microscopy (SEM), respectively. To study the in vitro degradation behavior, MPs were incubated in a phosphate buffered solution isotonized with NaCl (PBS, pH 7.4, 37ºC) at a constant agitation speed of 100 rpm. At different time points (1 hour, 24 hours, 48 hours and 5 days) MPs morphology was studied by SEM. In vitro tolerance studies were performed by the MTT technique in human corneal limbal epithelial cells and macrophage cells. Cells were exposed to MPs suspensions (5mg and 10mg MPs/ml in PBS) for 15 minutes (short term exposure), 1 hour and 4 hours (long term exposure). Dexamethasone (DX) was used as a lipophilic drug model to determine the encapsulation efficiency of PEA-II MPs (0.5:10 DX:PEAII).

Results: : Unloaded PEA-II MPs were spherical and had smooth surface. MPs size ranged between 10-30µm (mean particle size 21.3±0.2 µm). MPs started to lose their shape after being incubated in PBS for 1 hour. 5 days later, MPS had turned into an unshaped depot of polymer. The cytotoxicity assays demonstrated good tolerance in the two cell lines in all cases after short- and long-term exposures (cell viability>90%) at the assayed concentrations. DX-loaded MPs (9.62±0.56 µg DX/mg MPs and mean particle size 21.3±1.8 µm) did not show any morphological difference with unloaded MPs.

Conclusions: : Biodegradable PEA-II MPs are potentially useful to develop new controlled drug delivery systems for treating ophthalmic diseases.

Keywords: cell survival 

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