March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Superior Delivery to Choroid-Retina following Suprachoroidal Injections in Rats: Assessment using Fluorophotometry
Author Affiliations & Notes
  • Puneet Tyagi
    Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado
  • Rajendra S. Kadam
    Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado
  • Uday B. Kompella
    Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado
  • Footnotes
    Commercial Relationships  Puneet Tyagi, None; Rajendra S. Kadam, None; Uday B. Kompella, None
  • Footnotes
    Support  This work was supported by the NIH grants EY017533, EY018940, and EY017045.
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 487. doi:
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      Puneet Tyagi, Rajendra S. Kadam, Uday B. Kompella; Superior Delivery to Choroid-Retina following Suprachoroidal Injections in Rats: Assessment using Fluorophotometry. Invest. Ophthalmol. Vis. Sci. 2012;53(14):487.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The objective of this study was to evaluate the delivery and pharmacokinetics of sodium fluorescein after suprachoroidal, intravitreal, and posterior subconjunctival injections in Sprague Dawley rats using noninvasive fluorophotometry.

Methods: : Delivery and pharmacokinetics of sodium fluorescein was evaluated in Sprague Dawley rats (n=4) after suprachoroidal (500 ng in 5µl), intravitreal (50 ng in 5µl), or posterior subconjunctival injection (500 ng in 5µl). Sodium fluorescein levels were monitored noninvasively using Fluorotron Master™ up to 6 hours. Pharmacokinetic parameters were estimated by noncompartmental analysis using WinNonlin.

Results: : Sodium fluorescein delivery to choroid-retina was in the order: suprachoroidal > intravitreal > posterior subconjunctival injection. Peak concentration (Cmax; ng/ml) in the choroid-retina was the highest after suprachoroidal (1673 ± 363) injection when compared to intravitreal (103 ± 44) and posterior subconjunctival (76 ± 6) injections. The extent of delivery (AUC0-t) to the choroid-retina after suprachoroidal injection was 2- and 6- fold higher than intravitreal and posterior subconjunctival injections, respectively.

Conclusions: : Suprachoroidal injections resulted in the highest delivery of sodium fluorescein to choroid-retina when compared to intravitreal and posterior subconjunctival injections. Noninvasive monitoring of sodium fluorescein is possible in rats using Fluorotron Master™ ocular fluorophotometry.

Keywords: choroid • sclera 
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