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Puneet Tyagi, Rajendra S. Kadam, Uday B. Kompella; Superior Delivery to Choroid-Retina following Suprachoroidal Injections in Rats: Assessment using Fluorophotometry. Invest. Ophthalmol. Vis. Sci. 2012;53(14):487.
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The objective of this study was to evaluate the delivery and pharmacokinetics of sodium fluorescein after suprachoroidal, intravitreal, and posterior subconjunctival injections in Sprague Dawley rats using noninvasive fluorophotometry.
Delivery and pharmacokinetics of sodium fluorescein was evaluated in Sprague Dawley rats (n=4) after suprachoroidal (500 ng in 5µl), intravitreal (50 ng in 5µl), or posterior subconjunctival injection (500 ng in 5µl). Sodium fluorescein levels were monitored noninvasively using Fluorotron Master™ up to 6 hours. Pharmacokinetic parameters were estimated by noncompartmental analysis using WinNonlin.
Sodium fluorescein delivery to choroid-retina was in the order: suprachoroidal > intravitreal > posterior subconjunctival injection. Peak concentration (Cmax; ng/ml) in the choroid-retina was the highest after suprachoroidal (1673 ± 363) injection when compared to intravitreal (103 ± 44) and posterior subconjunctival (76 ± 6) injections. The extent of delivery (AUC0-t) to the choroid-retina after suprachoroidal injection was 2- and 6- fold higher than intravitreal and posterior subconjunctival injections, respectively.
Suprachoroidal injections resulted in the highest delivery of sodium fluorescein to choroid-retina when compared to intravitreal and posterior subconjunctival injections. Noninvasive monitoring of sodium fluorescein is possible in rats using Fluorotron Master™ ocular fluorophotometry.
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