March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Pathological Changes Of Hair Follicle And Meibomian Gland In Demodex Related Blepharitis
Author Affiliations & Notes
  • Wei Li
    Eye Inst & Xiamen Eye Ctr, Xiamen Univ Sch of Medicine, Xiamen, China
  • Yihui Mao
    Eye Inst & Xiamen Eye Ctr, Xiamen Univ Sch of Medicine, Xiamen, China
  • Yangluowa Qu
    Eye Inst & Xiamen Eye Ctr, Xiamen Univ Sch of Medicine, Xiamen, China
  • Jing Jie
    Eye Inst & Xiamen Eye Ctr, Xiamen Univ Sch of Medicine, Xiamen, China
  • Zhixing Geng
    Eye Inst & Xiamen Eye Ctr, Xiamen Univ Sch of Medicine, Xiamen, China
  • Hua Wang
    Eye Inst & Xiamen Eye Ctr, Xiamen Univ Sch of Medicine, Xiamen, China
  • Wensheng Chen
    Eye Inst & Xiamen Eye Ctr, Xiamen Univ Sch of Medicine, Xiamen, China
  • Hui He
    Eye Inst & Xiamen Eye Ctr, Xiamen Univ Sch of Medicine, Xiamen, China
  • Jie Yang
    Eye Inst & Xiamen Eye Ctr, Xiamen Univ Sch of Medicine, Xiamen, China
  • Zuguo Liu
    Medical College of Xiamen Univ, Eye Institute, Xiamen, China
  • Footnotes
    Commercial Relationships  Wei Li, None; Yihui Mao, None; Yangluowa Qu, None; Jing Jie, None; Zhixing Geng, None; Hua Wang, None; Wensheng Chen, None; Hui He, None; Jie Yang, None; Zuguo Liu, None
  • Footnotes
    Support  In part by grant No. 30872809 (LW) and 30872810 (LZ) from NSFC, CHINA
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 610. doi:
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      Wei Li, Yihui Mao, Yangluowa Qu, Jing Jie, Zhixing Geng, Hua Wang, Wensheng Chen, Hui He, Jie Yang, Zuguo Liu; Pathological Changes Of Hair Follicle And Meibomian Gland In Demodex Related Blepharitis. Invest. Ophthalmol. Vis. Sci. 2012;53(14):610.

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Abstract

Purpose: : Demodex inhabits in hair follicle, sebaceous gland and meibomian gland. More and more studies revealed high relevance of demodex in ocular surface diseases such as chronic blepharitis, onjunctivitis, and meibomian gland dysfunction. However, the exact pathogenic mechanism remains elusive. This study is subjected to investigate the pathogenic role of demodex on hair follicle and meibomian gland in blepharitis.

Methods: : Eyelashes from 20 patients of demodex blepharitis and 15 healthy volunteers, and eyelid tissue from 6 cadavers were studied. Apoptosis of hair follicle was determined by TUNEL assay. Ki67 and TUNEL double staining were performed on eyelid cross-sections. Epithelial differentiation of hair follicle and meibomian gland was determined by immunostaining of K10, K14, K15, and K16. Inflammatory cell infiltration was determined by CD11c and MAC387 immunostaining on eyelid tissues.

Results: : The apoptotic cells increased in hair follicle and surrounding tissue in demodex blepharitis. K10 expression extended into deep hair follicle, glandular tube and some acinus of the meibomian glands. K16 expression significantly expanded in hair follicle, while there was no significant change of K14 and K15 expression in the hair follicle. Demodex-positive tissues were infiltrated with abundant CD11c positive cells and MAC387 positive cells.

Conclusions: : Demodex induces hair follicle epithelial cell apoptosis, causes hyper-keratinization and hyper-proliferation of hair follicles, and abnormal keratinizatoin of meibomian gland, as well as inflammation of eyelid tissue.

Keywords: eyelid • apoptosis/cell death • pathobiology 
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