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Naoko Onizuka, Masafumi Uematsu, Mao Kusano, Takeshi Kumagami, Hitoshi Sasaki, Kiyoshi Suzuma, Takashi Kitaoka; Polyoxyethylene Hydrogenated Castor Oil Relieves The Acute Corneal Epithelial Toxicity Induced By Benzalkonium Chloride. Invest. Ophthalmol. Vis. Sci. 2011;52(14):300.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate whether polyoxyethylene hydrogenated castor oil (HCO40) reduces acute corneal epithelial toxicity induced by benzalkonium chloride (BAC).
The corneal barrier function was evaluated in vivo by measuring the corneal trans-epithelial electrical resistance (TER) in anesthetized Japanese white rabbits. An electrode was placed in the anterior aqueous chamber and another on the cornea; and the corneal TER was measured with a 20 µA current at 12.5 Hz using a volt-ohm meter (EVOMX). TER change from baseline was evaluated after 60 seconds of corneal exposure to 0.02% BAC alone or 0.02% BAC mixed with 0.01%, 0.1%, or 1% HCO40 (n=3).
Exposure to 0.02% BAC significantly lowered the corneal TER to 10+/-6% of the initial value. Adding 0.01% HCO40 did not significantly improve the corneal burrier function in the presence of BAC; at this concentration, the corneal TER was 11+/-5% of baseline. However, higher concentrations (0.1% and 1%) of HCO40 significantly alleviated the corneal epithelial barrier function disorder caused by 0.02%BAC in a concentration dependent manner; the respective corneal TERs were 50+/-10% and 96+/-4 % of baseline.
At higher concentrations, HCO40 reduces BAC toxicity in the cornea. When evaluating the toxicity of ophthalmic drugs, the ameliorating effect of additives such as HCO40 should be considered in conjunction with the BAC concentration.
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