April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Anti-Inflammatory And Anti-Oxidant Effects Of The Green Tea Polyphenol Epigallocatechin Gallate In Human Corneal Epithelial Cells
Author Affiliations & Notes
  • Karen L. Harrington
    Pharmaceutical R & D, Bausch + Lomb, Rochester, New York
  • Megan E. Cavet
    Pharmaceutical R & D, Bausch + Lomb, Rochester, New York
  • Thomas R. Vollmer
    Pharmaceutical R & D, Bausch + Lomb, Rochester, New York
  • Stepan M. Volhejn
    Pharmaceutical R & D, Bausch + Lomb, Rochester, New York
  • Jessica E. Ackerman
    Pharmaceutical R & D, Bausch + Lomb, Rochester, New York
  • Keith W. Ward
    Pharmaceutical R & D, Bausch + Lomb, Rochester, New York
  • Jin-Zhong Zhang
    Pharmaceutical R & D, Bausch + Lomb, Rochester, New York
  • Footnotes
    Commercial Relationships  Karen L. Harrington, Bausch + Lomb (E); Megan E. Cavet, Bausch + Lomb (E); Thomas R. Vollmer, Bausch + Lomb (E); Stepan M. Volhejn, Bausch + Lomb (E); Jessica E. Ackerman, Bausch + Lomb (E); Keith W. Ward, Bausch + Lomb (E); Jin-Zhong Zhang, Bausch + Lomb (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 311. doi:
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      Karen L. Harrington, Megan E. Cavet, Thomas R. Vollmer, Stepan M. Volhejn, Jessica E. Ackerman, Keith W. Ward, Jin-Zhong Zhang; Anti-Inflammatory And Anti-Oxidant Effects Of The Green Tea Polyphenol Epigallocatechin Gallate In Human Corneal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2011;52(14):311.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The aim of this study was to determine the anti-inflammatory and anti-oxidant effects of epigallocatechin gallate (EGCG), the major polyphenol component of green tea, in human corneal epithelial cells (HCEpiC).

Methods: : HCEpiC were challenged with IL-1β for 18 hours. Cell metabolic activity was measured using the alamarBlue assay and Luminex technology was used to determine the effects of EGCG (0.3 - 30 µM) on IL-1β-induced cytokine release (G-CSF, GM-CSF, IL-6, IL-8, and MCP-1) into the medium. Effects of EGCG on mitogen-activated protein kinase (MAPK) phosphorylation were determined by cell based ELISA and Western blotting. Effects of EGCG on nuclear factor kappa B (NFΚB) and activator protein-1 (AP-1) transcriptional activity were assessed by reporter gene assay. The effects of EGCG on glucose oxidase-induced reactive oxygen species (ROS) production was determined using the ROS probe CM-H2DCFDA.

Results: : Treatment of HCEpiC with 1 ng/ml IL-1β for 18 h significantly increased release of the cytokines/chemokines G-CSF, GM-CSF, IL-6, IL-8 and MCP-1. When cells were treated with IL-1β and EGCG there was a dose dependent reduction in release of these cytokines/chemokines, with significant inhibition observed at 3 - 30 µM. There was no effect of EGCG on cell metabolic activity at any of the doses tested (0.3 - 30 µM). Both 3 and 30 µM EGCG significantly inhibited phosphorylation of the MAPKs p38 and JNK, and NFΚB and AP-1 transcriptional activities. There was a significant dose dependent decrease in GO-induced ROS levels after treatment of HCEpiC with 3, 10 or 30 µM EGCG.

Conclusions: : EGCG acts as an anti-inflammatory and anti-oxidant agent in HCEpiC and therefore may have therapeutic potential for ocular inflamatory conditions such as dry eye.

Keywords: inflammation • cornea: epithelium • antioxidants 
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