April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Thin Of Corneal Epithelium In The Eye Of Perlecan-null Mice
Author Affiliations & Notes
  • Takenori Inomata
    Ophthalmology, Juntendo Univ School of Med, Bunkyo-ku,Tokyo, Japan
  • Toshinari Funaki
    Ophthalmology, Juntendo Univ School of Med, Bunkyo-ku,Tokyo, Japan
  • Nobuyuki Ebihara
    Ophthalmology, Juntendo Univ School of Med, Bunkyo-ku,Tokyo, Japan
  • Akira Matsuda
    Ophthalmology, Juntendo Univ School of Med, Bunkyo-ku,Tokyo, Japan
  • Yasuo Watanabe
    Ophthalmology, Juntendo Univ School of Med, Bunkyo-ku,Tokyo, Japan
  • Eri Arikawa-Hirasawa
    Neulorogy, Juntendo Univ Sch of Med, Bunkyo-ku,Tokyo, Japan
  • Akira Murakami
    Ophthalmology, Juntendo Univ School of Med, Bunkyo-ku,Tokyo, Japan
  • Footnotes
    Commercial Relationships  Takenori Inomata, None; Toshinari Funaki, None; Nobuyuki Ebihara, None; Akira Matsuda, None; Yasuo Watanabe, None; Eri Arikawa-Hirasawa, None; Akira Murakami, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 317. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Takenori Inomata, Toshinari Funaki, Nobuyuki Ebihara, Akira Matsuda, Yasuo Watanabe, Eri Arikawa-Hirasawa, Akira Murakami; The Thin Of Corneal Epithelium In The Eye Of Perlecan-null Mice. Invest. Ophthalmol. Vis. Sci. 2011;52(14):317.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Perlecan, a large, multi-domain, heparan sulfate proteoglycan originally identified in basement membrane, interacts with extracellular matrix proteins, growth factors and receptors, and influences cellular signaling. We reported small eye balls and the thin of corneal epithelium in the eye of perlecan-null mice by transmission electron microscopic analysis last year in ARVO. This study was performed to elucidate the roles of perlecan in the corneal epithelium of mice by histochemical analysis.

Methods: : The perlecan-null mice were generated as reported previously (Nat Genet. 23:354-8, 1999). We examined the 8week-old mice (Perlecan-null mice=5, control mice=5). The eyes of these mice were dissected and prepared for histological or molecular analysis. 3-µm paraffin sections were made from the perlecan-null mice and control mice. The specimens were stained with hematoxylin-eosin. For transmission electron microscopic analysis, tissues were fixed in 2.5% glutaraldehyde in Sorensen buffer, postfixed in osmium tetroxide. Subsequently, the corneal thickness measured under a microscope. We performed immunostaing of TUNEL, Ki67, p-63 and Cytokeratin12.

Results: : The ratio of the epithelial cell layer thickness to the full cornea thickness was 22.67±5.25%(mean±SD) in the perlecan-null mice group and 30.34±3.25% in the control group. The perlecan-null mice group showed the significant thinning of the corneal epithelium (p=0.0317). Immunostainng of Ki67, cell proliferation marker, in the perlecan-null group showed the significant reduction of the staining of nuclei(p=0.0343). On the other hand, immunostaing of TUNEL, p-63, and Cytokeratin12 showed no significant differences between the groups.

Conclusions: : Perlecan may affect the cell proliferation status in the corneal epithelium.

Keywords: cornea: epithelium • proteoglycans/glycosaminoglycans 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×