Purchase this article with an account.
Luo Luo Zheng, Yichieh Shiuey, Dale J. Waters, Phil Huie, Richard V. Manivanh, Jennifer R. Cochran, Curtis W. Frank, Christopher N. Ta; Artificial Lamellar Cornea Based On Poly(ethylene Glycol) And Poly(acrylic Acid). Invest. Ophthalmol. Vis. Sci. 2011;52(14):330.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To design an artificial lamellar cornea capable of supporting re-epithelialization and implantation without staged procedures or a donor corneal carrier.
A mechanically strong and nutrient-permeable hydrogel composed of an interpenetrating network of poly(ethylene glycol) and poly(acrylic acid) (PEG/PAA) was cast with a diameter of 7-mm and raised central button of 3-mm in diameter. After sterilization through autoclaving, the hydrogel was treated with chemical crosslinkers EDC and NHS to covalently bind bovine collagen Type I and bovine fibronectin to the hydrogel surface. For in vitro experiments, immortalized human corneal epithelial cells were seeded on top of the artificial cornea at a density of 2.5 x 10^4 cells/mL and cultured for two weeks. Molded hydrogels without epithelial cells were implanted into a stromal pocket in enucleated rabbit eyes created with the Keramed RoboTome Cornea Pocket Maker.
The surface-modified hydrogel supported a confluent growth of human corneal epithelial cells in culture. Cells stained positively for cytokeratin 3, indicating their corneal epithelial phenotype. The artificial cornea could be manipulated to fit into an 8-mm uniform circular stromal pocket created at a depth of 300 µm, such that the raised central button fit within the trephined anterior cornea.
An artificial lamellar cornea has been designed with a PEG/PAA hydrogel that possesses high mechanical strength and nutrient permeability, and has the potential to support re-epithelialization.
This PDF is available to Subscribers Only