April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Efficacy of Pig-to-Rhesus Lamellar Corneal Xenotransplantation
Author Affiliations & Notes
  • Mee Kum Kim
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Laboratory of corneal regenerative medicine and ocular immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • Hyuk Jin Choi
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Laboratory of corneal regenerative medicine and ocular immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • Hyun Ju Lee
    Laboratory of corneal regenerative medicine and ocular immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • So Hee Jeong
    Laboratory of corneal regenerative medicine and ocular immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • Won Ryang Wee
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Laboratory of corneal regenerative medicine and ocular immunology, Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Republic of Korea
  • Jin Hak Lee
    Ophthalmology, Seoul National University Bundang Hospital, Seongnam, Republic of Korea
  • Footnotes
    Commercial Relationships  Mee Kum Kim, None; Hyuk Jin Choi, None; Hyun Ju Lee, None; So Hee Jeong, None; Won Ryang Wee, None; Jin Hak Lee, None
  • Footnotes
    Support  a grant of the Korea Healthcare technology R & D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (Project No. A040004)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 363. doi:
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      Mee Kum Kim, Hyuk Jin Choi, Hyun Ju Lee, So Hee Jeong, Won Ryang Wee, Jin Hak Lee; Efficacy of Pig-to-Rhesus Lamellar Corneal Xenotransplantation. Invest. Ophthalmol. Vis. Sci. 2011;52(14):363.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the efficacy of pig-to-nonhuman primate lamellar corneal transplantation using both decellularized and fresh porcine corneas in order to assess feasibility as a substitute for human.

Methods: : Nine Chinese rhesus macaques underwent lamellar corneal transplantation using both decellularized (n=5) and fresh (n=4) porcine corneas. Minimal immunosuppression was applied using topical and systemic corticosteroid. Graft survival and immunologic profiles including T cell subset, anti-α-Gal/donor specific antibodies, and complement were evaluated.

Results: : 80% of decellularized porcine lamellar grafts remained transparent for more than 6 months while half of fresh porcine lamellar grafts developed chronic rejection. Immunologic profile showed enhancement of memory T cell in both groups, and variable increases in anti-α-Gal/donor specific antibodies case-by-case. Activated complement in the aqueous was significantly higher in recipients with rejected grafts compared than in recipients with survived grafts. CD8 effector T cells tend to be elevated in the recipients with rejected graft.

Conclusions: : Decellularized porcine cornea is likely to be a promising substitute to human corneal allograft. Xenogeneic corneal rejection may be associated with CD8 T cell and complement mediated reaction in primates.

Keywords: transplantation • cornea: basic science • cornea: clinical science 
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