April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Expression Of Lymphangiogenic Markers In Rejected Human Corneal Bed After Penetrating Keratoplasty
Author Affiliations & Notes
  • Hyung Keun Lee
    Ophthal-Severance Hosp,
    Yonsei Univ College of Medicine, Seoul, Republic of Korea
    Severance Institute of Vascular Metabolism Research, Yonsei Univeristy College of Medicine, Seoul, Republic of Korea
  • Eun Ju Chang
    Anatomy, School of Medicine, Ulsan University, Seoul, Republic of Korea
  • Mee-Kum Kim
    Ophthalmology, Seoul National Univ Hospital, Seoul, Republic of Korea
  • Soon Won Hong
    Yonsei Univ College of Medicine, Seoul, Republic of Korea
  • Eung-Kweon Kim
    Visual Science, Yonsei Univ College of Med, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Hyung Keun Lee, None; Eun Ju Chang, None; Mee-Kum Kim, None; Soon Won Hong, None; Eung-Kweon Kim, None
  • Footnotes
    Support  KRF-2010-0012899
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 371. doi:
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      Hyung Keun Lee, Eun Ju Chang, Mee-Kum Kim, Soon Won Hong, Eung-Kweon Kim; Expression Of Lymphangiogenic Markers In Rejected Human Corneal Bed After Penetrating Keratoplasty. Invest. Ophthalmol. Vis. Sci. 2011;52(14):371.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To investigate the mechanism of lymphangiogenesis in graft rejection process, we determined the expression level of several lymphangiogenic markers in rejected human corneal bed through immunohistochemical and molecular biologic works.

Methods: : Seventeen recipient corneas were secured from the patients who performed re-keratoplasty for graft rejection after penetrating keratoplasty. All corneas showed signs of active rejection such as Khodadoust line before re-keratoplasty. The corneas were cut in half to make two pieces of the same size and one piece was used for immunostaining and the other was used for RT-PCR for each corneas. To determine the lymphangiogenic markers, expression of vascular endothelial growth factor (VEGF)-A, VEGF-C, VEGF-D, VEGFR-2, VEGFR-3, fibroblast growth factor-2 (FGF-2), and LYVE-1 were investigated. Two normal corneas were included as control.

Results: : From the immunostaining of LYVE-1, we found many LYVE-1 expressed cells in anterior and posterior stroma and lymphatic vessels growing into the paracentral cornea. The expressions of VEGF-A, VEGFR-2, and LYVE-1 were upregulated in rejected cornea compared to the normal. In contrast, expressions of VEGF-C, VEGF-D, and VEGFR-3 were not changed in rejected graft. By the quantitative real time RT-PCR data, mRNA for VEGF-A, VEGFR2 expression ratio (keratoplastic cornea/normal cornea) was 8.2 in VEGF-A, 3.9 in VEGFR2. However, mRNA for VEGF-C, VEGF-D, and VEGF3 were 1.3, 0.9, and 1.2, respectively. FGF-2 did not show the increment in rejected corneal bed.

Conclusions: : These findings may suggest that VEGF-A and VEGFR2 axis is more important pathway for corneal graft rejection and lymphangiogenesis than VEGF-C, -D, or VEGFR3 axis.

Keywords: cornea: basic science • immune tolerance/privilege • vascular endothelial growth factor 

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