Purchase this article with an account.
Sandeep S. Samudre, Brian Philippy, David Korroch, Bruce Bodner; Efficacy Comparison of Optisol GSTM and Life 40CTM Corneal Storage Media. Invest. Ophthalmol. Vis. Sci. 2011;52(14):378.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Although Optisol GSTM is the widely used storage media for short term storage of corneas, corneal viability and performance once transplanted decline after a few days of storage, as demonstrated by swollen endothelial cells and increased corneal thickness. Life4ºCTM, a new corneal storage preservation media containing insulin, is hypothesized to enhance cell survival. In this preliminary study, we aim to determine how Life4ºCTM compares to Optisol GSTM with respect to maintenance of healthy endothelial cell density and prevention of corneal swelling.
After procurement, corneas with endothelial cell density between 2000 to 3500 cells/mm2 were selected for the study and were assigned randomly to either Optisol GSTM or Life4ºCTM. Donor selection criteria include: age range of 5 - 65 years, phakic, no refractive surgery or keratoplasty history, a death to preservation interval maximum of 12 hours, and a death to cooling interval maximum of 10 hours. Assessments, including specular microscopy, pachymetry (corneal thickness) and slit-lamp examinations (SLE), were performed by a masked observer at baseline and after 24 hours and 36 hours from preservation date. Data is presented as mean ± SEM.
Cell density of corneas preserved in Life4ºCTM was 2682±51 cells/mm2 at baseline, and was not affected after 24 hours, 2652±51 cells/mm2, and after 36 hours, 2525±119 cells/mm2 (p=0.4, n=3). Similarly, cell density of corneas preserved in Optisol GSTM was 2769±92 cells/mm2 at baseline, and was not affected after 24 hours, 2731±153 cells/mm2 and after 36 hours, 2624±25 cells/mm2 (p=0.6, n=3). Corneal thickness was also not affected after preservation in Life4ºCTM as the baseline of 553±7 µm, was similar to 535±15 µm after 24 hours and 539±17 µm after 36 hours (p=0.6, n=3). Similarly, preservation in Optisol GSTM did not affect corneal thickness, as baseline of 562±13µm, was similar to 556±8 µm after 24 hours and 540±7 µm after 36 hours (p=0.3, n=3). Although mild cell dropout was observed in both groups, SLE was within normal limits.
In this preliminary study, after 36 hour preservation, Life4ºCTM was comparable to Optisol GSTM in terms of cell density, pachymetry and SLE. No significant endothelial cell drop out was observed in both storage media. Long-term studies comparing Life4ºCTM to Optisol GSTM are currently underway.
This PDF is available to Subscribers Only