April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Accumulation of Lipid Peroxidation Products in Optisol GS and Organ Culture Storage Media prior to Human Corneal Transplantation
Author Affiliations & Notes
  • Kristiane Haug
    Center for Eye Reseach, Department of Ophthalmology, Oslo University Hospital, Ullevål, Oslo, Norway
  • Siv Johnsen-Soriano
    Fundación Oftalmológica del Mediterráneo (FOM), Valencia, Spain
  • Emma Arnal
    Fundación Oftalmológica del Mediterráneo (FOM), Valencia, Spain
  • Bjørn Nicolaissen
    Center for Eye Reseach, Department of Ophthalmology, Oslo University Hospital, Ullevål, Oslo, Norway
  • Javier Francisco Romero
    Fundación Oftalmológica del Mediterráneo (FOM), Valencia, Spain
    Universidad CEU Cardenal Herrera, Valencia, Spain
  • Footnotes
    Commercial Relationships  Kristiane Haug, None; Siv Johnsen-Soriano, None; Emma Arnal, None; Bjørn Nicolaissen, None; Javier Francisco Romero, None
  • Footnotes
    Support  NILS mobility project, Universidad Complutense de Madrid, Spain, Blindemissionen IL, Norwegian Association for the Blind and Partially Sighted, Oslo University Hospital Ullevaal, University of Oslo.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 379. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Kristiane Haug, Siv Johnsen-Soriano, Emma Arnal, Bjørn Nicolaissen, Javier Francisco Romero; Accumulation of Lipid Peroxidation Products in Optisol GS and Organ Culture Storage Media prior to Human Corneal Transplantation. Invest. Ophthalmol. Vis. Sci. 2011;52(14):379.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Optisol GS and Organ Culture medium are currently used as storage media for donor corneas. Little is known about potential alterations that may occur in these two media during corneal storage prior to transplantation. The purpose of the study was to examine the level of the oxidative stress marker malondialdehyde (MDA) in the upper and lower layers of the storage media, Optisol GS and Organ Culture, during storage of corneas for transplantation.

Methods: : Sixty human corneas were stored either in Optisol GS or in Organ culture medium, for 7-14 days prior to transplantation. At different times (0, 7 and 14 days) medium was taken out form the upper and lower layers of the storage containers, and MDA were measured by HPLC.

Results: : Measurement of the lipid peroxidation product MDA in the upper and lower layers of Optisol GS and Organ Culture medium, showed higher amounts of MDA in lower layers when compared to upper layers (p < 0,05), as well as increasing levels of MDA at longer storage time (p < 0,05).

Conclusions: : Measurements of MDA in Optisol GS and Organ Culture medium after 0, 7, and 14 days revealed increasing levels during storage and a higher accumulation in the lower layers of the medium. This observation calls for a closer examination of donor cornea storage systems devices and their influence on cell damage during storage.

Keywords: cornea: storage 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×