Purchase this article with an account.
Manabu Hirasawa, Kousuke Noda, Misa Suzuki, Yoko Ozawa, Kazuo Tsubota, Susumu Ishida; Cytokine Regulation of Transcriptional Factor Snail in Human Retinal Pigment Epithelium. Invest. Ophthalmol. Vis. Sci. 2011;52(14):413.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
We previously reported the upregulation and nuclear localization of Snail, representative transcriptional factor for epithelial-mesenchymal transition (EMT), in retinal pigment epithelium (RPE) of choroidal neovascularization from patients with age-related macular degeneration (AMD) (ARVO 2010). In this study, we investigated the cytokine regulation on Snail expression in cultured human RPE cells.
ARPE19, a human RPE cell line, was used in this study. ARPE19 cells were stimulated with representative proinflammatory or proangiogenetic cytokines, TGF (transforming growth factor) -beta, tumor-necrosis factor (TNF)-alpha, vascular endothelial growth factor (VEGF) and connective tissue growth factor (CTGF), at the confluent state. Cellular localization and expression level of Snail in ARPE19 were analyzed by immunofluorescence microscopy and reverse transcription-polymerase chain reaction.
Snail was found mainly in cytoplasm of ARPE19 cells at the confluent state. Immunofluorescence microscopy depicted the enhanced staining of Snail in the nucleus and subsequent fibroblast-like change of ARPE-19 cells stimulated with TGF-beta, indicating the nuclear relocalization of Snail and EMT change. Furthermore, TGF-beta and VEGF significantly upregulated Snail expression in ARPE19 cells.
The current data indicate that TGF-beta plays a role in EMT change of RPE cells via upregulation and nuclear relocalization of Snail, suggesting a novel mechanism in the pathogenesis of AMD.
This PDF is available to Subscribers Only