Purchase this article with an account.
Irene Bravo-Osuna, Noiray Magali, Ashley Woodward, Pablo Argüeso, Rocio Herrero-Vanrell, Irene Teresa Molina-Martinez, Rafael Gómez, FJ de la Mata, Manuel Guzmán Navarro, Gilles Ponchel; Carboxilane Dendrimers For Ophthalmic Administration. Mucoadhesion studies. Invest. Ophthalmol. Vis. Sci. 2011;52(14):424.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To evaluate the interaction between purified ocular surface mucins and carbosilane dendrimers-generation1, 2 & 3-, intended for drug delivery in ophthalmology, using Biosensor technology
Ocular surface mucins were purified from stratified cultures of human corneal limbal epithelial (HCLE) cells using size-exclussion chromatogaphy followed by isopycnic density gradient centrifugation. Surface plasmon resonance (SPR) technique was chosen to evaluate the interaction of dendrimers with those ocular mucins. Saline solutions of carbosilane dendrimers (5-10 µM) were passed over the sensor chip, where mucins were previously immobilized, for 300s at 10 µl/min. Afterwards, the sensorgram was collected until equilibrium was reached. Studies were performed in triplicate at 35ºC.
As determined by western blot using the M11 antibody against MUC16, purified samples from HCLE cells consisted of mucin with a buoyant density range of 1.26 to 1.44 g/ml. Anionic dendrimers showed SPR sensorgrams indicative of non-molecular adhesion. A significant part of cationic dendrimers put in contact with mucins were retained in the mucin layer. The intensity of this interaction was statistically higher for G3 (18 NH2 surface groups), where 66 dendrimer molecules appeared associated to one mucin molecule, in comparison to the G2 (12 NH2) and G1 (6 NH2), where the dendrimer-mucin ratio was 27:1 and 29:1 respectively.
Results demonstrate the deep interaction of carbosilane dendrimers with ocular mucins and support the utility of dendrimers as new drug delivery systems for topical ophthalmic drug administration.
This PDF is available to Subscribers Only