April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
A Novel Microfabricated Instrument to Treat Recurrent Corneal Erosions
Author Affiliations & Notes
  • Daniel K. Bennett
    Ophthalmology, University of Tennessee - Memphis, Memphis, Tennessee
  • Xiaofei Wang
    Ophthalmology, University of Tennessee - Memphis, Memphis, Tennessee
  • John T. Simpson
    Oak Ridge National Laboratory, Oak Ridge, Tennessee
  • Robert J. Warmack
    Oak Ridge National Laboratory, Oak Ridge, Tennessee
  • Edward Chaum
    Ophthalmology, University of Tennessee - Memphis, Memphis, Tennessee
  • Footnotes
    Commercial Relationships  Daniel K. Bennett, None; Xiaofei Wang, None; John T. Simpson, Author (P); Robert J. Warmack, Author (P); Edward Chaum, Author (P)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 426. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Daniel K. Bennett, Xiaofei Wang, John T. Simpson, Robert J. Warmack, Edward Chaum; A Novel Microfabricated Instrument to Treat Recurrent Corneal Erosions. Invest. Ophthalmol. Vis. Sci. 2011;52(14):426.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

To evaluate the efficacy of a novel instrument in the treatment of recurrent corneal erosions (RCEs). Current treatment of RCEs includes conservative management as well as mechanical techniques, including epithelial debridement and anterior stromal puncture. These methods can induce significant scarring via injury to Bowman’s membrane and disruption of laminar stromal architecture. A microfabricated glass-based corneal instrument was designed and tested ex vivo to investigate a new technique for anterior stromal puncture that provides reproducible penetration into the corneal stroma with minimal visually significant scarring.


Experiments were performed using a microfabricated glass corneal instrument providing a 3-dimensional array of designable spikes on a hand-held platform. The spikes measure 60-70µm in length with a pitch of 120µm. Soft contact lenses and porcine corneas were used, divided into multiple sections and indented using the corneal punch. Mechanical disruption of the substrate and India ink served as identifying markers for imaging.


The India ink and mechanical patterns in the lens and corneal sections revealed regular penetration of the array into the anterior corneal stroma. Coating the spikes with India ink showed reliable and reproducible stromal deposition of the dye from the matrix array.


The microfabricated corneal instrument reliably penetrated corneal stroma in several trials on porcine corneas and soft contact lenses with excellent reproducibility. The benefit of the corneal punch is a patterned matrix that provides an attachment point for basal epithelial cells by inducing microscopic scars at each point. The regular spacing and small width of the spikes creates focal defects and avoids unnecessary damage and potential visual loss via visual, refractive, or anatomical complications seen with other forms of anterior stromal puncture. The instrument may also serve as a novel delivery system for medications targeted to corneal stroma and those unable to penetrate intact corneal epithelium.  

Keywords: cornea: clinical science • cornea: epithelium • cornea: stroma and keratocytes 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.