April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Controlled Vancomycin Release from Chondroitin Sulfate - Polyethylene Glycol (CS-PEG) Tissue Adhesive for Treatment of Corneal Injuries
Qiaozhi Lu; Jemin J. Chae; Qiongyu Guo; Iossif Strehin; Mehnaz Khan; Freddy A. Espinoza; Jianan Zhan; Oliver Schein; Morgana M. Trexler; Jennifer H. Elisseeff
Author Affiliations & Notes
  • Qiaozhi Lu
    Department of Biomedical Engineering,
    Johns Hopkins University, Baltimore, Maryland
  • Jemin J. Chae
    Department of Biomedical Engineering,
    Johns Hopkins University, Baltimore, Maryland
  • Qiongyu Guo
    Department of Biomedical Engineering,
    Johns Hopkins University, Baltimore, Maryland
  • Iossif Strehin
    Department of Biomedical Engineering,
    Johns Hopkins University, Baltimore, Maryland
  • Mehnaz Khan
    Vanderbilt School of Medicine, Nashville, Texas
  • Freddy A. Espinoza
    Department of Biomedical Engineering,
    Johns Hopkins University, Baltimore, Maryland
  • Jianan Zhan
    Department of Biomedical Engineering,
    Johns Hopkins University, Baltimore, Maryland
  • Oliver Schein
    Department of Ophthalmology,
    Johns Hopkins University, Baltimore, Maryland
  • Morgana M. Trexler
    Milton Eisenhower Research Center, Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland
  • Jennifer H. Elisseeff
    Department of Biomedical Engineering,
    Johns Hopkins University, Baltimore, Maryland
  • Footnotes
    Commercial Relationships  Qiaozhi Lu, None; Jemin J. Chae, None; Qiongyu Guo, None; Iossif Strehin, None; Mehnaz Khan, None; Freddy A. Espinoza, None; Jianan Zhan, None; Oliver Schein, None; Morgana M. Trexler, None; Jennifer H. Elisseeff, None
  • Footnotes
    Support  DoD Eye Patch Grant 90042163
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 439. doi:
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      Qiaozhi Lu, Jemin J. Chae, Qiongyu Guo, Iossif Strehin, Mehnaz Khan, Freddy A. Espinoza, Jianan Zhan, Oliver Schein, Morgana M. Trexler, Jennifer H. Elisseeff; Controlled Vancomycin Release from Chondroitin Sulfate - Polyethylene Glycol (CS-PEG) Tissue Adhesive for Treatment of Corneal Injuries. Invest. Ophthalmol. Vis. Sci. 2011;52(14):439.

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Abstract

Purpose: : To create a dual-purpose ocular repair material for corneal injuries while releasing antibiotics to prevent post-traumatic infection.

Methods: : In vitro vancomycin release. Vancomycin free base (V-FB) was prepared from vancomycin hydrochloride (V-HCl). To make 100 µl hydrogel, 20% w/v CS-NHS and PEG-(NH2)6 were mixed in equal volumes and 2% V-FB or V-HCl was loaded. Hydrogels were immersed in 1 ml PBS and incubated at 37ºC (n=3). PBS fractions were collected at various time points and the concentration of vancomycin was analyzed by HPLC. Kirby-Bauer test was performed to evaluate the activity of vancomycin released.In vivo application. To evaluate the material-tissue interaction, rabbit corneas (n=6) with or without injuries were treated with a 20% CS-PEG adhesive. Two types of corneal injuries were created with 8 mm Hessburg-Barron vacuum trephines: partial thickness defect and deepithelialization. Flurosecein dye and H&E staining were used for observing tissue adhesion and local tissue response.

Results: : In vitro vancomycin release. HPLC results demonstrated that vancomycin was successfully released from CS-PEG. The cumulative release profile of V-FB was significantly more sustained than that of V-HCl, which produced a burst release of 82% over the first 24 hours, compared to 68% for V-FB. Considering the mass released, V-FB exhibited a more stable trend. In day 3, the mass released from V-FB form was approximate 1.5-fold of the corresponding V-HCl form. Kirby-Bauer tests confirmed that the vancomycin released from both forms was functional.In vivo application. CS-PEG applied on both deep and superficial corneal injury models adhered well to the local tissue and served as an ocular adhesive. It remained on injured corneal tissue over 7 days, which is adequate for release of antibiotics, as determined in the in vitro study. In the uninjured group, where epithelium was intact, the adhesive detached within 1 day. Thus it is notable that only the damaged areas would bind with CS-PEG, and no attachment was found on the other areas of the cornea.

Conclusions: : In vivo and in vitro data supports the efficacy of the biosynthetic CS-PEG ocular tissue adhesive formulation for the application to treat corneal injuries and release of vancomycin.

Keywords: antibiotics/antifungals/antiparasitics • wound healing • cornea: basic science 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
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