April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Silicone-coated Superparamagnetic Nanoparticles Citotoxicity On Two Distinct Ocular Cell Population
Author Affiliations & Notes
  • Joao C. Grottone
    Ophthalmology, UNIFESP, Santos SP, Brazil
  • Gustavo T. Grottone
    Ophthalmology, UNIFESP, Sao Paulo, Brazil
  • Jose Alvaro P. Gomes
    Ophthalmology, UNIFESP, Sao Paulo, Brazil
  • Renata R. Loureiro
    Ophthalmology, UNIFESP, Sao Paulo, Brazil
  • Footnotes
    Commercial Relationships  Joao C. Grottone, None; Gustavo T. Grottone, None; Jose Alvaro P. Gomes, None; Renata R. Loureiro, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 444. doi:
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      Joao C. Grottone, Gustavo T. Grottone, Jose Alvaro P. Gomes, Renata R. Loureiro; Silicone-coated Superparamagnetic Nanoparticles Citotoxicity On Two Distinct Ocular Cell Population. Invest. Ophthalmol. Vis. Sci. 2011;52(14):444.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Evaluate citotoxicity and magnetic response of different ocular cell populations cultured under silicon-coated superparamagnetic nanoparticle solution

Methods: : ARPE-19 cells and human corneal endothelial cells were divided in three different groups. Group A received culture media plus sham. Group B received culture media plus SPIO(ferumoxsil 300 nm) at a concentration of 0.21 mg Fe/ml. Group C received culture media plus SPIO(ferumoxsil 300 nm) at a concentration of 2.1 mg Fe/ml. Citotoxicity was measured after 24 hours incubation by XTT method. After XTT method was performed, cells were released from the culture dishes with collagenase A 2mg/ml and resulting aggregates were tested against a 3500 gauss Neodymium Magnet. Kruskal-Wallis and Dunn tests were used comparing the three groups on each cell type.

Results: : The citotoxicity evaluation by XTT method, showed a higher toxicity at the groups tested with Group C(2.1 mg Fe/ml)p<0.05.Groups A and B had similar results with a different variance between groups(not significant). No significant difference was noticed between the different cell types(ARPE-19 and HCEC). The magnetic attraction of these different cell types was similar on both concentrations used. Group B and C had an efficiency of 100% of aggregates been attracted by an external magnetic field. Control group A had no magnetic attraction in comparison with other test groups. The two different cell types kept the same response under magnetic stimuli.

Conclusions: : Same behaviour against magnetic challenge was noticed between different cell types. The method used for resuspension of cultured cells, encapsulated a large amount of SPIO nanoparticles within cell aggregates. At the two different concentrations tested, the lower concentration of 0.21 mg Fe/ml seems to have a lower citotoxicity and similar magnetic response when comparing to the 2.1mg Fe/ml group. Other studies should be performed to clarify the inter and intracellular presence of this ferumoxsil nanoparticles.

Keywords: retinal pigment epithelium • cell survival • transplantation 
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