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Joao C. Grottone, Gustavo T. Grottone, Jose Alvaro P. Gomes, Renata R. Loureiro; Silicone-coated Superparamagnetic Nanoparticles Citotoxicity On Two Distinct Ocular Cell Population. Invest. Ophthalmol. Vis. Sci. 2011;52(14):444.
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Evaluate citotoxicity and magnetic response of different ocular cell populations cultured under silicon-coated superparamagnetic nanoparticle solution
ARPE-19 cells and human corneal endothelial cells were divided in three different groups. Group A received culture media plus sham. Group B received culture media plus SPIO(ferumoxsil 300 nm) at a concentration of 0.21 mg Fe/ml. Group C received culture media plus SPIO(ferumoxsil 300 nm) at a concentration of 2.1 mg Fe/ml. Citotoxicity was measured after 24 hours incubation by XTT method. After XTT method was performed, cells were released from the culture dishes with collagenase A 2mg/ml and resulting aggregates were tested against a 3500 gauss Neodymium Magnet. Kruskal-Wallis and Dunn tests were used comparing the three groups on each cell type.
The citotoxicity evaluation by XTT method, showed a higher toxicity at the groups tested with Group C(2.1 mg Fe/ml)p<0.05.Groups A and B had similar results with a different variance between groups(not significant). No significant difference was noticed between the different cell types(ARPE-19 and HCEC). The magnetic attraction of these different cell types was similar on both concentrations used. Group B and C had an efficiency of 100% of aggregates been attracted by an external magnetic field. Control group A had no magnetic attraction in comparison with other test groups. The two different cell types kept the same response under magnetic stimuli.
Same behaviour against magnetic challenge was noticed between different cell types. The method used for resuspension of cultured cells, encapsulated a large amount of SPIO nanoparticles within cell aggregates. At the two different concentrations tested, the lower concentration of 0.21 mg Fe/ml seems to have a lower citotoxicity and similar magnetic response when comparing to the 2.1mg Fe/ml group. Other studies should be performed to clarify the inter and intracellular presence of this ferumoxsil nanoparticles.
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